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Pakistan Journal of Nutrition

Year: 2010 | Volume: 9 | Issue: 5 | Page No.: 471-474
DOI: 10.3923/pjn.2010.471.474
Isolation, Characterization and Production of Phytase from Endophytic Fungus its Application for Feed
Yetti Marlida, Rina Delfita, Peri Adnadi and Gita Ciptaan

Abstract: Thirty four isolates of endophytic fungus produce phytases were isolated from leaf, stem and root fragments of soybean. Two isolates were the best of phytases enzyme producer and identified as Rhizoctonia sp. and Fusarium verticillioides. The phytase production was induced by phytate in medium used. The crude preparations were used in subsequent characterization studies, pH and temperature optimum and compared to other phytases tested and is thus a promising candidate for animal feed applications. The results showed that optimal production of phytase from Rhizoctonia sp. were pH 4.0 and temperature 50oC and pH 5.0, temperature 50oC for Fusarium verticillioides.

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How to cite this article
Yetti Marlida, Rina Delfita, Peri Adnadi and Gita Ciptaan, 2010. Isolation, Characterization and Production of Phytase from Endophytic Fungus its Application for Feed. Pakistan Journal of Nutrition, 9: 471-474.

Keywords: Endophytic fungus, phytase, soybean, Rhizoctonia sp. and Fusarium verticillioides

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