Abstract: This research work was carried out to compare the physicochemical parameters of milk samples of four different species like buffalo, cow, goat and sheep. Milk samples were collected from the different areas of Gujrat, Pakistan and analyzed for different physiochemical parameters, including pH, specific gravity, titratable acidity, total solids, ash, fat, protein and lactose. It was recorded that buffalo milk had 6.75±0.15 pH, 1.033±0.002 specific gravity, 0.21±0.03% titratable acidity, 18.45±0.85% total solids, 0.81±0.09% ash, 7.97±0.44% fat and 4.36±0.23% protein and 5.41±0.54% lactose. Cow milk had 6.64±0.02 pH, 1.029±0.001 specific gravity, 0.17±0.02% titratable acidity, 12.94±0.97% total solids, 0.60±0.13% ash, 4.00±0.43% fat, 3.37±0.32% protein and 4.51±0.38% lactose. Goat milk had 6.55±0.06 pH, 1.030±0.001 specific gravity 0.16±0.01% titratable acidity, 12.84±0.56% total solids, 0.75±0.13% ash, 3.97±0.51% fat, 3.15±0.32% protein and 4.39±0.34% lactose. Sheep milk contained 6.63±0.04 pH, 1.034±0.002 specific gravity, 0.23±0.01% titratable acidity, 18.13±0.21% total solids, 0.88±0.07% ash, 6.49±0.23% fat, 5.30±0.29% protein and 4.77±0.31% lactose. All the tested parameters were higher in buffalo and sheep milk than cow and goat milk.