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Pakistan Journal of Biological Sciences

Year: 2006 | Volume: 9 | Issue: 1 | Page No.: 123-127
DOI: 10.3923/pjbs.2006.123.127
Effects of Aerobic and Anaerobic Incubation On the Quality of Ram Semen
M. U. Samo, R.F. E. Axford, T. A. Qureshi, A. A. Memon and M. M. Memon

Abstract: Effects of aerobic and anaerobic incubation on the quality of ram semen were investigated. The semen was assessed applying Activity index, Motility % (PLM), Swim-up test, Osmotic Resistance Test (ORT) and Oxygen utilization of the cells. Methylene blue crystals were used as an indicator for oxygen utilization of the cells. Usage of Methylene blue crystals was found useful as an indicator for oxygen utilization. It was observed that the incubation conditions had a significant (p = 0.05) effect on the quality of the cells. Activity of the cells had no relationship with intactness of the cell membrane as the cells with broken membrane were found motile in ORT test. Time of incubation also exerted a significant (p = 0.05) effect on the quality of the semen. It is concluded that the semen should be used within 2 h, either fresh or frozen-thawed and supplied with a sufficient quantity of a suitable energy source in the dilution medium.

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How to cite this article
M. U. Samo, R.F. E. Axford, T. A. Qureshi, A. A. Memon and M. M. Memon, 2006. Effects of Aerobic and Anaerobic Incubation On the Quality of Ram Semen. Pakistan Journal of Biological Sciences, 9: 123-127.

Keywords: Ram, spermatozoa, metabolism, incubation, aerobic and anaerobic

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