Influence of pH on the ATPase Activity and Solubility of Macrobrachium
rosenbergii and Penaeus monodon Muscle Myofibrillar Protein
Abstract:
The influence of pH on the changes in myofibrillar remaining ATPase
activities of M. rosenbergii and P. monodon was studied after
storage at -20°C for 2 days, 0°C for 2 days and 35°C for 30 min. In all the
three storage conditions, ATPase activities for both samples were lower in
acidic and alkaline pH regions and the activity remains relatively high of 0.403
° mol Pi/min mg at pH 8.1. The influence of pH on the remaining Mg2+-ATPase
activity and Ca-sensitivity of M. rosenbergii and P. monodon were
evaluated after storage at 0°C for 2 days and -20°C for 2 days. Mg2+-ATPase
activities both in presence and absence of Ca2+ remain high at
neutral pH compared to those of acidic and alkaline region. Ca-sensitivity were
also very high (about 38 to 50% at pH ranges from 7.4 to 8.3) in neutral pH
region where the sensitivity declined sharply both in acidic and alkaline pH
region. The influence of pH on the changes in solubility of M. rosenbergii
muscle myofibrils during ice and frozen storage was investigated. The
highest solubility of 83 to 84% was obtained from myofibrils in the range of pH
7.4 to pH 7.9 after storage for 2 days in ice and -20°C for 2 days in frozen
storage. The solubility decreased gradually both in acidic and alkaline pH
regions. In the case of P. monodon, the maximum solubility of 84% were
obtained at pH 7.9 after 2 days of storage at 0°C. Similarly the maximum
solubility of 79% were obtained at same pH of 7.8 in myofibrils after storage at
-20°C for 2 days. The solubility of P. monodon myofibrils was quite high
in a wide range of pH from 6.8 to 8.3 and the solubility decreased gradually
outside of these pH ranges. Comparatively higher solubility of 84% in a wide
range of pH in myofibrils stored at 0°C compared to that of frozen storage also
indicates the combined effect of pH and frozen storage on the denaturation of
muscle myofibrils.
How to cite this article
Md. Kamal, Md. Moshiur Rahman, Mohammad Abu Jafor Bapary, Lubna Yasmin and Md. Nurullah, 2005. Influence of pH on the ATPase Activity and Solubility of Macrobrachium
rosenbergii and Penaeus monodon Muscle Myofibrillar Protein. Pakistan Journal of Biological Sciences, 8: 982-988.
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