HOME JOURNALS CONTACT

Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 1 | Page No.: 78-84
DOI: 10.3923/pjbs.2005.78.84
The Effect of Coelomic Fluid Stichopus hermanii on Isolated Perfused Rat Hearts and the Involvement of Prostaglandin in its Mechanism of Action
W. T. Tan, Yew Su Fong and B. H. Ridzwan

Abstract: The objective of this study was to investigate the effect of the coelomic fluid (freeze-dried) on the contractility, heart rate and coronary perfusion pressure of the isolated perfused rat hearts by using the Langendorff system. The second objective of the study was to investigate the involvement of prostaglandin in the mechanism action of the fluid. Isoprenaline (ISO) and sodium nitroprusside (SNP) were used as positive controls. The dose-response curve for ISO was determined in which 1.0 nmol ISO produced maximum contractility and heart rate. Then the effect of the coelomic fluid was determined followed by its dose-response curve. The fluid showed vasorelaxation effect on coronary arteries but not on the heart rate and contractility. The dose 1.0 mg fluid was chosen in the mechanistic study since it produced maximum vasorelaxation and this effect was similar to 70% effect of SNP (1x10-6 mol). The mechanistic studies were divided into 3 parts. First, the time-matched control study. The result showed two identical doses (1.0 mg) of the fluid injected repeatedly into the heart gave similar effect. This study being used as a standard for further mechanism studies. The second and third parts, followed similar protocol as in the time-matched control. The first dose of the fluid was injected into the heart perfused with Kreb-Henseleit solution without adding Indomethacin (cyclooxygenase inhibitor). The second dose was injected into the heart perfused with the solution added Indomethacin (0.01 mM). The result showed Indomethacin reduced the vasorelaxation effect of the fluid by 52%. In conclusion, the studies indicated showed the coelomic fluid of S. hermanii caused vasorelaxation effect on coronary arteries and the mechanism involved prostaglandin.

Fulltext PDF

How to cite this article
W. T. Tan, Yew Su Fong and B. H. Ridzwan, 2005. The Effect of Coelomic Fluid Stichopus hermanii on Isolated Perfused Rat Hearts and the Involvement of Prostaglandin in its Mechanism of Action. Pakistan Journal of Biological Sciences, 8: 78-84.

Keywords: Sea cucumber, Stichopus hermanii, role on isolated rat heart, contractility, heart rate, coronary perfussion pressure, mechanism, coelomic fluid and prostagladin

REFERENCES
  • Ridzwan, B.H. and C.C. Che Bashah, 1985. Tinjauan awal taburan dan penilaian holothurian di beberapa kawasan perairan. Sabah sebagai sumber pemakanan. Sumber, 1: 143-155.

  • Nihayah, M., R. Yanti, A.R. Nur Afiah, A.B. Mohd Azman and B.H. Ridzwan, 2000. Kesan penyembuhan ekstrak Stichopus variegatus ke atas lesi gaster teraruh tikus. Proceedings of the Pascasidang Simposium Sains Kesihatan Kebangsaan Ke-3, (PSSKK`00), FSKB, UKM, Kuala Lumpur, pp: 259-263.

  • Ridzwan, B.H., M.Z.Z., Kaswandi, M.A.M. Nadirah and A.F. Shamsuddin, 2001. The antinociceptive effects of extracts from Stichopus chloronotus brandt. Pak. J. Biol. Sci., 4: 244-246.
    CrossRef    Direct Link    

  • Ridzwan, B.H., M.A. Kaswandi, Y. Azman and M. Fuad, 1995. Screening for antibacterial agents in three species of sea cucumber from coastal areas of Sabah. Gen. Pharmacol., 26: 1539-1543.
    CrossRef    PubMed    Direct Link    

  • Fredalina, B.D., B.H. Ridzwan, A.A. Zainal Abidin, M.A. Kaswandi and H. Zaiton et al., 1999. Fatty acid compositions in local sea cucumber, Stichopus chloronotus, for wound healing. Gen. Pharmacol., 33: 337-340.
    CrossRef    Direct Link    

  • Chiew, T.S.B., 2001. Mekanisme vasodilatasi oleh ekstrak kayu balak Hopea odorata pada jantung terpisah tikus. M.Sc. Thesis, FSKB, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia.

  • Rahman, A., R. Husain and M.A. Muhammad, 1995. Fisiology Manusia. 2nd Edn., Shah Alam, Publisher, Fajar Bakti, Kuala Lumpur, Malaysia

  • Steward, A.G. and P.J. Piper, 1988. Vasodilator action of acetylcholine, A23187 and bradykinin in guinea-pig isolated perfused heart are independent of prostacyclin. Br. J. Pharmacol., 95: 379-384.
    PubMed    Direct Link    

  • Amenta, M., L. Coppola and P. Gallo, 1991. Autoradiographic localization of beta-adrenergic receptors in human large coronary arteries. Circ. Res., 68: 1591-1599.
    Direct Link    

  • Edmunds, N.M. and B. Woodward, 1998. Effects of tumour necrosis factor on the coronary circulation of the isolated perused heart: a potential role for thromboxane A2 and spingosine. Br. J. Pharmacol., 114: 1745-1749.

  • Norton, J.M. and R. Dexter, 1972. Potassium and isolated coronary vascular smooth muscle. Am. J. Physiol., 222: 474-479.
    Direct Link    

  • Haddy, F.J., 1983. Potassium effect on contraction in arteriole smooth muscle mediated by sodium and potassium ions. Federal Proc., 42: 55-59.

  • Katzung, B.G., 1998. Basic and Clinical Pharmacology. 7th Edn., Appleton and Lange, Stanford CT., pp: 372-375

  • Koller, A. and A. Huang, 1999. Development of nitric oxide and prostaglandin mediation for shear stress-induced arteriolar dilation with aging and hypertension. Hypertension, 34: 1073-1079.
    Direct Link    

  • Opie, L.H., 1997. The Heart: Physiology, from Cell to Circulation. 3rd Edn., Lippincott-Raven, Philadelphia

  • Campbell, W.B. and P.V. Halushka, 1995. Lipid-derived Autocoids: Eicosanoids and Platelet Activating Factor. In: Goodman and Gillman`s the Pharmacological Basis of Therapeutic, Hardman J.G. and L.E. Limbird (Eds.). 9th Edn., McGraw Hill, New York, pp: 601-616

  • Schror, K., S. Moncada, F.B. Ubatuba and J.R. Vane, 1978. Transformation of arachidonic acid and prostaglandin endoperoxides by the guinea pig heart. Formation of RCS and prostacyclin. Eur. J. Pharmacol., 47: 103-114.
    Direct Link    

    • © Science Alert. All Rights Reserved