Abstract: The investigation was carried out to evaluate the shelf life of Pangus (Pangasius hypopthalamus) stored in ice in relation to size and season. Samples collected from different ghers in Khulna region of Bangladesh during April 2002 to January 2003 were assessed both in summer and winter for three sizes of fish (large, medium and small). Quality deterioration was studied for 18 days by organoleptic and biochemical (TVB-N, TMA-N and pH) parameters. In summer, for large fish, the keeping time varied between 10 to 12 days when the value of TVB-N, TMA-N and pH ranged between 17.88 ± 0.97 to 24.12± 0.08, 4.43± 1.47 to 5.23± 1.05 mg N/100 g and 6.75± 0.50 to 7± 0.08; while the acceptability limit in winter was the 10th day. Within this period, the value of TVB-N, TMA-N and pH was 17.04 ± 1.02, 4.03 ± 0.84 mg N/100 g and 6.64± 0.09, respectively. In case of medium fish during summer, the shelf life was 12 days when the value of TVB-N, TMA-N and pH was 22.46± 1.36, 5.47 ± 0.54 mg N/100 g and 6.8 ± 0.08. The shelf life in winter season was found on the 10th day. At that time, the value of TVB-N, TMA-N and pH was 15.08 ± 0.06, 4.78 ± 0.84 mg N/100 g and 6.2 ± 0.04. For small fish, in summer, the rejection time was on the 10th day and the value of TVB-N, TMA-N and pH was 19.71 ± 1.05, 4.61 ± 0.74 mg N/100 g and 6.86 ± 0.05. However in winter, the just acceptability limit was found on the 10th day. The value of TVB-N, TMA-N and pH was 18.32 ± 0.58, 4.52 ± 0.68 mg N/100 g and 6.78 ± 0.50 during this period.