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Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 4 | Page No.: 546-549
DOI: 10.3923/pjbs.2005.546.549
Estimation of Genetic Diversity of Four Chrysanthemum Mini Cultivars Using RAPD
J. Chatterjee, A. K. Mandal, S. A. Ranade and S. K. Datta

Abstract: The objective of the present study was to estimate the genetic relationship among the different Chrysanthemum cultivars with relation to their morphological and biochemical characteristics and geographical distribution. DNA fingerprinting using RAPD is very easy and inexpensive way to study the genetic diversity. Genetic distance between four mini Chrysanthemum cultivars was studied through RAPD analysis. Total of 40 primers have been screened from which four have been trailed for all the four genotypes. Similarity among the cultivars was very high showing low genetic diversity, which is quite expected. One of these primers can differentiate cultivars from each other. So RAPD can be used successfully to estimate the genetic distance and also for the species identification.

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How to cite this article
J. Chatterjee, A. K. Mandal, S. A. Ranade and S. K. Datta, 2005. Estimation of Genetic Diversity of Four Chrysanthemum Mini Cultivars Using RAPD. Pakistan Journal of Biological Sciences, 8: 546-549.

Keywords: Genetic diversity, chrysanthemum miniature cultivars and RAPD

REFERENCES
  • Demeke, T., R.P. Adums and R. Chibbar, 1992. Potential taxonomic use of RAPD a case study in Brassica. Theor. Applied Genet., 84: 990-994.
    CrossRef    

  • Hu, J.G. and C.F. Quiros, 1991. Identification of broccoli and cauliflower cultivars with RAPD markers. Plant Cell Rep., 10: 505-511.
    CrossRef    

  • Kresovich, S., J.G.K. Williams, J.R. McFerson, E.J. Routman and B.A. Schaal, 1992. Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor. Applied Genet., 85: 190-196.
    CrossRef    PubMed    Direct Link    

  • Hallden, C., N.O. Nilsson, I.M. Rading and T. Sall, 1994. Evaluation of RFLP and RAPD markers in a comparison of Brassica napus breeding lines. Theor. Applied Genet., 88: 123-128.
    CrossRef    Direct Link    

  • Mailer, R.J., R. Scarth and B. Fnstensky, 1994. Discrimination among cultivars of rapeseed (Brassica napus L.) using DNA polymorphisms amplified from arbitrary primers. Theor. Applied Genet., 87: 697-704.
    CrossRef    Direct Link    

  • Wolff, K., J.P. Van Rijn and H. Hofstra, 1994. RFLP analysis in chrysanthemum probe and primer development. Theor. Applied Genet., 88: 472-478.
    CrossRef    

  • Schroeder, K.R., D.P. Stimart and E.V.J. Northeim, 2001. Estimation of total chlorophyll from peach cultivars. Am. Hortic. Sci., 125: 523-530.

  • Saghai-Maroof, M.A., K.M. Soliman, R.A. Jorgensen and R.W. Allard, 1984. Ribosomal DNA spacer-length polymorphisms in barly: Mendelian inheritance, chromosomal location, and population dynamics. Proc. Natl. Acad. Sci., 81: 8014-8018.
    CrossRef    Direct Link    

  • Nei, M. and W.H. Li, 1979. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA., 76: 5269-5273.
    CrossRef    PubMed    Direct Link    

  • Sneath, P.H.A. and R.R. Sokal, 1973. Numerical Taxonomy. 1st Edn., W.H. Freeman and Co., San Francisco, USA., ISBN-10: 0716706970, Pages: 573

  • Nunes, M.J.C., M.A. Machado, W.M.C. Nunes, M. Cristofani and M.L.P. Targon, 2002. Assesment of genetic variability in grapefruits (Citrus paradisi Macf.) and pummelos (C. maxima (Burm.) Merr.) using RAPD and SSR markers. Euphytica, 126: 169-176.

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