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Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 3 | Page No.: 418-420
DOI: 10.3923/pjbs.2005.418.420
Evaluation of RT-PCR for Rapid Detection of Sudanese Isolates and Vaccine Strains of Newcastle Disease Virus
Mohamed A.M. Yousof, I. E. Aradaib, K.M. S. Khairalla, M. A. Abdalla, A.R. E. Karrar and A.R. M. ElHussein

Abstract: A Reverse Transcriptase (RT) Polymerase Chain Reaction (PCR) was developed to detect field isolates of Newcastle Disease Virus (NDV) in vero cell culture or Embryonated Chicken Eggs (ECE). Five Sudanese isolates of NDV designated (OB, KU, GR, A12 and A105) and five vaccine strains including Komarov, B1, LaSota, Clon30 and Clon79 were used in this study. A pair of primers (ND1 and ND2), targeting a fragment in the F gene of NDV, was designed for PCR amplification. The RT-PCR assay resulted in amplification of a 356 bp PCR product from RNAs of Sudanese and vaccine strains of NDV. However, nucleic acid extracts of Infectious Bursal Disease (IBD) virus, non infected vero cells or ECE failed to produce the specific 356 bp PCR product. The described RT-PCR assay was a simple procedure that involved a single amplification step. In addition, the developed RT-PCR assay provides a rapid, sensitive and specific method for detection of an out break of the disease in susceptible birds.

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How to cite this article
Mohamed A.M. Yousof, I. E. Aradaib, K.M. S. Khairalla, M. A. Abdalla, A.R. E. Karrar and A.R. M. ElHussein, 2005. Evaluation of RT-PCR for Rapid Detection of Sudanese Isolates and Vaccine Strains of Newcastle Disease Virus. Pakistan Journal of Biological Sciences, 8: 418-420.

Keywords: Newcastle disease virus, molecular diagnostics and RT-PCR

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