HOME JOURNALS CONTACT

Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 2 | Page No.: 284-286
DOI: 10.3923/pjbs.2005.284.286
Correlation Between Microscopic Examination and Culture for Detection and Differentiation of Mycobacterial Isolates from Cattle in the Sudan
S. H. Manal, M. E. Hamid, I.M. El Jalii and A. S. Ali

Abstract: One hundred and sixty seven caseated lymph nodes and tuberculous lungs were collected from cattle slaughtered in various locations in Khartoum State (Sudan) and examined bacteriologically. Microscopic examination using Zeilh-Neelsen stain and culture on Lowenstein-Jensen (L-J) medium were carried out to detect and differentiate between the mycobacterial species in the specimens. Thirty-five, 35 (20.96%) samples were found to harbor acid-fast bacteria when examined microscopically. Out of the 35 acid-fast bacteria, 22 (62.86%) showed branching filaments and were identified as Mycobacterium farcinogenes. The remaining 13(37.14%) were bacilli and identified as Mycobacterium bovis. The 35 specimens that proved to harbor acid fast bacteria were cultured on L-J medium. None of the 22 specimens with branching filaments (Mycobacterium farcinogenes) grew on L-J medium when incubated aerobically at 37 ° C between four and eight weeks. 12 (92.31%) of the 13 bacilli (Mycobacterium bovis) showed visible growth using the above growth conditions. In conclusion, microscopic examination can only detect acid fast organisms in the clinical samples whereas culture on L-J medium can differentiate between acid-fast mycobacterial species.

Fulltext PDF

How to cite this article
S. H. Manal, M. E. Hamid, I.M. El Jalii and A. S. Ali, 2005. Correlation Between Microscopic Examination and Culture for Detection and Differentiation of Mycobacterial Isolates from Cattle in the Sudan. Pakistan Journal of Biological Sciences, 8: 284-286.

Keywords: Mycobacteria, microscopy and culture

REFERENCES
  • Blood, D.C., O.M. Radostits and J. Henderson, 1986. Veterinary Medicine a Textbook of Diseases of Cattle, Sheep, Pigs, Goats and Horses. 6th Edn., Bailliere Tindall, London, pp: 631-643

  • Wayne,.L.G. and G.B. Kubica, 1986. Genus Mycobacterium. In: Bergers Manual of Systemic Bacteriology, Sneath, P.H.H., N.S. Mair, M.E. Sharpe and J.G. Holt (Eds.). Williams and Wilkins, Baltimore MD., pp: 1436-1457

  • Daborn, C.J. and J.M. Grange, 1993. HIV/AIDS and its implications for the control of animal tuberculosis. Br. Vet. J., 149: 405-417.
    Direct Link    

  • Fabrizio V., C. Giuseppina, M. Letizia, R. Stefano, V. Gesualdo and C. Santo, 1998. Detection of Mycobacterium tuberculosis complex in cattle by PCR using milk, lymph node aspirates and nasal swabs. J. Clin. Microbiol., 36: 1050-1055.
    Direct Link    

  • Cowan, S.T. and K.J. Steel, 1965. Manual for the Identification of Medical Bacteria. Cambridge University Press, Cambridge, UK

  • Notle, F.S. and B. Mitchock, 1995. Mycobacterium. In: Manual of Clinical Microbiology, Murray, P.R. and E.J. Boroan (Eds.). 6th Edn., American Society for Microbiology, Washington, DC., pp: 400-440

  • Quinn, P.J., M.E. Carter, B. Markey and G.R. Carter, 1994. Clinical Veterinary Microbiology. 1st Edn., Mosby-Yearbook Europe Ltd., UK.

  • Tageldin, M.H.A., 1971. Comparative study of the pathology and bacteriology of human and animal tuberculosis in the Sudan. Ph.D. Thesis, University of Khartoum

  • Robert, G.D., E.W. Konemon and Y.K. Kim, 1991. Mycobacterium. In: Manual of Clinical Microbiology, Balows, A., W.J. Hausler, J.R. K.I. Herman, H.D. Isenbery and H.J. Shadomy (Eds.). 5th Edn., American Society for Microbiology, Washington, DC., pp: 304-339

  • Sulieman, M.S., 2000. Identification of acid-fast bacteria isolated from slaughtered cattle in the Sudan. M.Sc. Thesis, University of Khartoum.

  • Lesslie, I.W., 1959. A comparison of biological and some cultural methods for the primary isolation of Mycobacterium tuberculosis. J. Comp. Pathol., 69: 1-10.

  • Sulieman, M.S. and M.S. Hamid, 2002. Identification of acid-fast bacteria from caseous lesions in cattle in Sudan. J. Vet. Med., 49: 415-418.
    Direct Link    

  • Corner, L.A., 1981. The duration of the response of cattle to inoculation with atypical mycobacteria. Aust. Vet. J., 57: 216-219.
    Direct Link    

  • Jorgensen, J.B., 1981. Pathogenicity and immunogenicity of atypical mycobacteria for calves a short summary. Rev. Infect. Dis., 3: 979-980.
    Direct Link    

  • Charles, O.T., 1997. Mycobacterium Bovis Infection in Cattle. In: Animal Tuberculosis in Africa and Middle East/Tuberculose Animale en Afrique Etau Moyen, Berrada, J., N. Bouchriti and M. Bouslikhane (Eds.). Orient Co., Pakistan, pp: 55-61

    • © Science Alert. All Rights Reserved