Anaerobic Biotransformation of Guaiacol to Catechol by Growing and Non-growing Harvested Cells of Acetobacterium woodii
DSM 1030
Abstract:
Acetobacterium woodii, an anaerobic acetogenic bacterium was used in this study to investigate the biotransformation
of guaiacol in batch culture via O-demethylation reaction. The culture was grown anaerobically using 250 mL conical flask
containing guaiacol in the presence or the absence of fructose. Results showed that limiting fructose concentration for the
highest cell growth was 2.0 g L-1. It is also found that 94% of guaiacol was transformed to catechol (0.56 g
L-1) when 2.0 g L-1 of fructose and 0.6 g L-1 of guaiacol were used as carbon source for
growth. In cultures containing 2 g L-1 fructose (with addition of 0.6 g L-1 guaiacol after 20 h
incubation), the catechol production was 0.5 g L-1 (82% transformation), while in cultures containing only
guaiacol, the catechol production was 0.4 g L-1 (62% transformation). In the experiment using harvested cells, the
highest catechol production was 0.47 g L-1 (79% transformation). The highest transformation was obtained using
non-growing harvested cells from cultures containing 2 g L-1 fructose with 0.6 g L-1 guaiacol addition
suggesting that this is the best combination of carbon source for high production of catechol. It is also suggested that high
cell concentration in culture containing limited concentration of fructose will resulted in high concentration of catechol
believed to be due of stimulation of the enzyme for O-demethylation reaction.
How to cite this article
Mohd Sahaid Kalil, Zainatul Asyiqin Shamsuddin and Noorhisham Tan Kofli, 2005. Anaerobic Biotransformation of Guaiacol to Catechol by Growing and Non-growing Harvested Cells of Acetobacterium woodii
DSM 1030. Pakistan Journal of Biological Sciences, 8: 1663-1666.
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