Abstract: This study aimed to determine the antimicrobial activity of crude extracts of three Palestinian folkloric medicinal plants and some of their subfractions in addition to their commercial oils on certain pathogenic microbes. Leaves of sage, thyme and parsley were collected, dried and extracted with different solvents to yield eight extracts. Three concentrations of each plant extracts were prepared. Disk diffusion methods were used to evaluate the antimicrobial activities of the extracts against ten different pathogenic microorganisms. The aqueous extracts of sage were, generally, of broad action against most of the tested microorganisms. All other extracts, showed some activities against one or more microorganisms. Sage, thyme and parsley extracts showed no antimicrobial effect on E. coli. In general, thyme extracts showed low effect on the tested Gram negative bacteria. However, parsley extract showed low effect on both Gram positive and negative bacteria. Among the ten tested microorganisms, Enterococcus sp. was the most susceptible microbe to extracts from sage and thyme plants. It is recommended that sage and thyme extracts may be used for food preservation, as well as, pharmaceutical and natural plant based products.