Abstract: In this study the susceptibility status of the field and laboratory strains of Anopheles stephensi, the main malaria vector, was determined to different larvicides. Five larvicides, i.e., chlorpyrifos-methyl, Bacillus thuringiensis, temephos, fenitrothion and methoprene were tested using WHO standard test method in the laboratory condition, against the field and lab strains of Anopheles stephensi Liston of Hormozgan province, southeastern Iran. The LC50 values were calculated from the probit-regression line for each larvicide. Results exhibited the LC50 values for B. thuringiensis, chlorpyrifos-methyl, fenitrothion, temephos and methoprene as 0.08483, 0.01115, 0.001131, 0.001613, 0.00073 mg L-1, respectively for lab strain. The values of 0.521279, 0.016419, 0.002475, 0.003388 and 0.000825 mg L-1, were measured as LC50 against field strain, respectively. From the results it can be concluded that there was significant difference between two strains for B. thuringiensis, temephos and fenitrothion at the LC50 level. The field strain was more tolerant than lab strain to the three larvicides. At diagnostic dose as recommended by WHO both strains are susceptible to all larvicides, therefore they can be applied for malaria vector control in the region.