Immuno Histochemistry of Cholesterol Cleft Granulomas in Non-specific Interstitial Pneumonia
Abstract:
This study aimed to define the cell populations involved in the granulomas. The granulomas of 16 patients with cryptogenic fibrosing alveolitis (five cases with the histological features of Non-specific Interstitial Pneumonial (NSIP), five with those of UIP and six cases of respiratory bronchiolitis) were examined histologically and by the use of immunohistochemical markers. Granulomas were discrete, compact and present only in alveolar spaces. The granulomas contained central clefts surrounded by mononuclear and multinucleated giant cells, both of which were CD68 positive. The cells outside the granulomas and those lining the adjacent alveolar walls were AE1/AE3 and CAM5.2 positive and CD68 negative. Our observations indicate that cholesterol cleft granulomas occur with high frequency in NSIP, are present within alveolar spaces which are lined exclusively by type II pneumocytes and that external to this the interstitium is fibrotic with accumulations of mast cells. It is our speculation that these are linked in a pathogenetic mechanism related to the progression of NSIP. The results also suggest that the mononuclear and multinucleated cells of cholesterol cleft granulomas are derived from the macrophage-mononuclear cell lineage. The alveolar lining cells are type II pneumocytes which do not contribute to the granuloma cell population. The alveolar lining cells are type II pneumocytes which do not contribute to the granuloma cell population.
How to cite this article
Abolfazl Barkhordari, R. W. Stoddart and Sheena F. Mclureand John Mcclure, 2005. Immuno Histochemistry of Cholesterol Cleft Granulomas in Non-specific Interstitial Pneumonia. Pakistan Journal of Biological Sciences, 8: 1383-1386.
REFERENCES
Maheshwari, U., D. Gupta, A.N. Aggarwal and S.K. Jindal, 2004. Spectrum and diagnosis of idiopathic pulmonary fibrosis. Indian J. Chest. Dis. Allied Sci., 46: 23-26.
Direct Link
Selman, M., V.J. Thannickal, A. Pardo, D.A. Zisman, F.J. Martinez and J.P. Lynch, 2004. III Idiopathic pulmonary fibrosis pathogenesis and therapeutic approaches. Drugs, 64: 405-430.
PubMed
Williams, K., D. Malarkey, L. Cohn, D. Patrick, J. Dye and G. Toews, 2004. Identification of spontaneous feline idiopathic pulmonary fibrosis morphology and ultrastructural evidence for a type II pneumocyte defect. Chest, 125: 2278-2288.
Direct Link
Flaherty, K.R., W.D. Travis, T.V. Colby, G.B. Toews and F.J. Martinez et al., 2001. Histopathologic variability in usual and nonspecific interstitial pneumonias. Am. J. Respir. Crit. Care. Med., 164: 1722-1727.
Direct Link
Takahashi, H., T. Fujishima, H. Koba, S. Murakami and S. Abe et al., 2000. Serum surfactant proteins A and D as prognostic factors in idiopathic pulmonary fibrosis and their relationship to disease extent. Am. J. Respir. Crit. Care. Med., 162: 1109-1114.
Direct Link
Katzenstein, A.L.A. and J.L. Myers, 1998. Idiopathic pulmonary fibrosis clinical relevance of pathologic classification. Am. J. Respir. Crit. Care. Med., 157: 1301-1315.
Katzenstein, A.L.A. and R.F. Fiorelli, 1994. Nonspecific interstitial pneumonia fibrosis histological features and clinical significance. Am. J. Surg. Pathol., 18: 136-147.
Cottin, V., A.V. Donsbeck, D. Revel, J.F. Loire and J.F. Cordier, 1998. Non specific interstitial pneumonia individualization of a clinicopathologic entity in a series of 12 patients. Am. J. Respir. Crit. Care. Med., 158: 1286-1293.
Guesdon, J.L., T. Ternynck and S. Avrameas, 1979. The use of avidin-biotin interaction in immunoenzymatic techniques. J. Histochem. Cytochem., 27: 1131-1139.
Waddell, W.R., R.C. Sniffen and L.L. Whytehead, 1949. Chronic pneumonitis: Its clinical and pathological importance. J. Thorac. Surg., 28: 134-144.
Navasquez, S.D. and G.A.D. Haslewood, 1954. Endogenous lipoid pneumonia with special reference to carcinoma of the lung. Thorax, 9: 35-37.
Reid, J.D., A.P. Oliver and P.C. Cairney, 1961. Cholesterol pneumonitis. N.Z. Med. J., 60: 134-143.
Corrin, B. and S.S. Soliman, 1973. Cholesterol in the lungs of heavy cigarette smokers. Thorax, 33: 565-568.
Yu, S., P.G. Harding, N. Smith and F. Possmayer, 1983. Bovine pulmonary surfactant chemical composition and physical properties. Lipids, 18: 522-529.
PubMed
Chambers, T.J. and W.G. Spector, 1982. Nflammatory giant cells. Immunobiology, 161: 283-289.
Warfel, A.H. and J.W. Hadden, 1978. Macrophage fusion factor elicited from BCG sensitized lymphocytes. Am. J. Pathol., 93: 753-770.
McNally, A.K. and J.M. Anderson, 1995. Interleukin 4 induces foreign body giant cells from human monocytes macrophages differential lymphokine regulation of macrophage fusion leads to morphological variants of multinucleated giant cells. Am. J. Pathol., 147: 1487-1499.
Direct Link
Fife K.M.D., C.R. Jenney, A.K. McNally, E. Colton and J.M. Anderson, 1997. Interleukin 13 induces human monocyte macrophage fusion and macrophage mannose receptor expression. J. Immunol., 158: 3385-3390.
Direct Link
Fenhalls, G., A. Wong, J. Bezuidenhout, P.V. Helden, P. Bardin and P.T. Lukey, 2000. In situ production of gamma interferon, interleukin 4 and tumor necrosis factor alpha mRNA in human lung tuberculous granulomas. Infect. Immun., 68: 2827-2836.
Direct Link
Barkhordari, A., R.W. Stoddart, S.F. McClure and J. McClure, 1999. Cellular glycotypes in pulmonary sarcoidosis. J. Pathol., 189: 24-24.
Barkhordari, A., R.W. Stoddart, S.F. McClure and J. McClure, 2000. Lectin binding profiles of pneumocytes in normal human lung. J. Pathol., 190: 54-54.
Barkhordari, A., R.W. Stoddart, S.F. McClure and J. McClure, 2004. Lectin histochemistry of normal human lung. J. Mol. Histol., 35: 147-156.
CrossRef
© Science Alert. All Rights Reserved