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Pakistan Journal of Biological Sciences

Year: 2005 | Volume: 8 | Issue: 1 | Page No.: 132-135
DOI: 10.3923/pjbs.2005.132.135
Effect of Fenfluramine on Molybdenum Hydroxylases Activities in Guinea Pigs
Yousuf Abdulmalik Al-Tayib

Abstract: The effects of fenfluramine on the activities of molybdenum hydroxylases, aldehyde oxidase and xanthine oxidase, activities in the liver, kidney, small intestine and lung of guinea pigs has been investigated. Pre-treatment of guinea pigs with fenfluramine (10 mg/kg/day) for 7 days resulted in a statistically significant decrease (p<0.0005, p<0.03) in activity of liver, small intestine and lung aldehyde oxidase using phthalazine, phenanthridine and 3-methylisoquinoline as substrates. Liver and small intestine xanthine oxidase of fenfluramine treated guinea pigs was also found a statistically significant decrease in its activity (p<0.0005) using xanthine as a substrate. The Km value for phthalazine which was also a significant decrease (p<0.005) and demonstrated regarding hepatic aldehyde oxidase from fenfluramine-treated guinea pig, as compared to the phenanthridine and 3-methylisoquinoline Km values which also revealed no significant change. In addition to that, no significant change to the km value of xanthine was recorded with hepatic xanthine oxidase from fenfluramine-treated guinea pigs.

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How to cite this article
Yousuf Abdulmalik Al-Tayib , 2005. Effect of Fenfluramine on Molybdenum Hydroxylases Activities in Guinea Pigs. Pakistan Journal of Biological Sciences, 8: 132-135.

Keywords: Fenfluramine, molybdenum hydroxylases, aldehyde oxidase and xanthine oxidase

REFERENCES
  • Chaney, S.G., 1997. Principle of Nutrition. In: Textbook of Biochemistry, Devlin, T.M. (Ed.). Wiley, New York, pp: 1094-1095

  • Pinder, R.M., R.N. Brogden, P.R. Sawyer, T.M. Speight and G.S. Avery, 1975. Fenfluramine: A review of its pharmacological properties and therapeutic efficacy in obesity. Drugs, 10: 241-323.
    PubMed    Direct Link    

  • Alsieni, A.I., A.O. Abuelgassim and S.M. Khoja, 1999. Effect of fenfluramine on the activities of key enzymes of the glycolytic pathway in rats. Med. Sci. Res., 27: 531-534.
    Direct Link    

  • Turtle, J.R. and J.A. Burgess, 1973. Hypoglycemic action of fenfluramine in diabetes mellitus. Diabetes, 22: 858-867.
    PubMed    Direct Link    

  • Altomonte, L., A. Zoli, G. Ghirlanda, R. Mann and A.V. Greco, 1988. Effects of fenfluramine on insulin/growth hormone ratio in obese subjects. Pharmacology, 36: 106-111.
    CrossRef    Direct Link    

  • Al-Sieni, A.I., C.P. Pested, Y. Rolland and D.N. Brindley, 1989. Decreased incorporation of glucose into lipids and increased lactate production by adipose tissue after long-term treatment of rats with D-fenfluramine. Biochem. Pharmacol., 38: 3661-3667.
    PubMed    Direct Link    

  • Johnson, C., C. Beedham and J.G.P. Stell, 1987. Reaction of 1-amino and 1-chlorophthalazine with mammalian molybdenum hydroxylases in vitro. Xenobiotica, 17: 17-24.
    PubMed    Direct Link    

  • Al-Tayib, Y., 1999. The circadian variation of the activity of hepatic molybdenum hydroxylases in the female Syrian hamster. Arab Gulf J. Sci. Res., 17: 370-381.
    Direct Link    

  • Johnson, C., C. Stubley-Beedham and J.G.P. Stell, 1984. Elevation of molybdenum hydroxylase levels in rabbit liver after ingestion of phthalazine or its hydroxylated metabolite. Biochem. Pharmacol., 33: 3699-3705.

  • Johnson, C., C.S. Beedham and J.G.P. Stell, 1985. Hydralazine: A potent inhibitor of aldehyde oxidase activity in vitro and in vivo. Biochem. Pharmacol., 34: 4251-4256.

  • Beedham, C., D.J. Padwick, Y. Al-Tayib and J.A. Smith, 1989. Diurnal variation and melatonin induction of hepatic molybdenum hydroxylases activity in guinea pig. Biochem. Pharmacol., 38: 1459-1464.
    PubMed    Direct Link    

  • Gornall, A.G., C.J. Bardawill and M.M. David, 1949. Determination of serum proteins by means of the biuret reaction. J. Biol. Chem., 177: 751-766.
    CrossRef    PubMed    Direct Link    

  • Krenitsky, T.A., S.M. Neil, G.B. Elion and G.H. Hitchings, 1972. A comparison of the specificities of xanthine oxidase and aldehyde oxidase. Arch. Biochem. Biophys., 150: 585-599.

  • Beedham, C., Y. Al-Tayib and J.A. Smith, 1992. Role of guinea pig and rabbit hepatic aldehyde oxidase in oxidative in vitro metabolism of cinchona antimalarials. Drug Metab. Disposit., 20: 889-895.
    Direct Link    

  • Beedham, C., D.J.P. Critchley and D.J. Rance, 1995. Substrate specificity of human liver aldehyde oxidase toward substituted quinazolines and phathazines: A comparison with hepatic enzyme from guinea pig, rabbit and baboon. Arch. Biochem. Biophys., 319: 481-490.

  • Critchley, D.J., D.J. Rance and C. Beedham, 1992. Subcellular localisation of guinea pig hepatic molybdenum hydroxylases. Biochem. Biophys. Res. Commun., 185: 54-59.
    Direct Link    

  • Ohkubo, M., S. Sakyama and S. Fujimura, 1983. Purification and characterisation of N1-methylnicotinamide oxidases 1 and 2 separated from rat liver. Arch. Biochem. Biophys., 221: 534-542.
    PubMed    Direct Link    

  • Holmes, R.S., 1978. Electrophoretic analysis of alcohol dehydrogenase, aldehyde dehydrogenase, aldehyde oxidase, sorbitol dehydrogenase and xanthine oxidase. Biochem. Physiol., 61B: 339-346.
    PubMed    Direct Link    

  • Gluecksohn-Waelsch, S., P. Greengard, G.P. Quinn and L.S. Teicher, 1967. Genetic variations of an oxidase in mammals. J. Biol. Chem., 242: 1271-1273.
    Direct Link    

  • Huff, S.D. and S. Chaykin, 1967. Genetic and androgenic control of N1-methylnicotinamide oxidase activity in mice. J. Biol. Chem., 242: 1265-1270.
    Direct Link    

  • Holmes, R.S., 1979. Genetics, ontogeny and testosterone inducibility of aldehyde isozymes in the mouse: Evidence for two genetic loci (AOX-1 and AOX-2) closely linked on chromosome 1. Biochem. Genet., 17: 517-527.
    PubMed    Direct Link    

  • Holmes, R.S., L.R. Leijten and J.A. Duley, 1981. Liver aldehyde oxidase and xanthine oxidase genetics in the mouse. Anim. Blood Groups Biochem. Genet., 12: 193-199.

  • Levinson, D.J. and D. Chalker, 1980. Rat hepatic xanthine oxidase activity: Age and sex specific differences. Arthritis Rheum., 23: 77-82.
    PubMed    Direct Link    

  • Blundell, J.E. and C.L. Lawton, 1995. Serotonin and dietary fat intake: Effects of dexfenfluramine. Metabolism, 44: 33-37.
    PubMed    Direct Link    

  • Geller, E., E.R. Ritvo, B.J. Freeman and A. Yuwiler, 1982. Preliminary observations on the effect of fenfluramine on blood serotonin and symptoms in three autistic boys. New Engl. J. Med., 307: 165-169.
    PubMed    Direct Link    

  • Ho, A.K. and J.A. Smith, 1982. Effect of benserazide on the levels of 5-hydroxytryptamine, melatonin synthesis enzymes and serum melatonin. Biochem. Pharmacol., 31: 2251-2255.
    PubMed    Direct Link    

  • Itoch, M.T., B. Ishizuka, Y. Kuribayashi, A. Amemiya and Y. Sumi, 1999. Melatonin, its precursors and synthesizing enzyme activities in the human ovary. Mol. Hum. Reprod., 5: 402-408.
    Direct Link    

  • Hamada, T., M. Ootomi, K. Horikawa, T. Niki, H. Wakamatu and N. Ishida, 1999. The expression of the melatonin synthesis enzyme: Arylalkylamine N-acetyltransferase in the suprachiasmatic nucleus of rat brain. Biochem. Biophys. Res. Commum., 258: 772-777.
    CrossRef    Direct Link    

  • Abe, M., M.T. Itoh, M. Miyata, S. Ishikawk and Y. Sumi, 1999. Detection of melatonin, its precursors and related enzyme activities in rabbit lens. Exp. Eye Res., 68: 252-262.
    Direct Link    

  • Al-Tayib, Y.A., 2004. The effect of melatonin on molybdenum hydroxylases activities of kidney and small intestine of guinea pigs. J. Egypt. Ger. Soc. Zool., 43A: 203-213.

  • Beedham, C., 1985. Molybdenum hydroxylases as drug metabolizing enzymes. Drug Metab. Rev., 16: 119-156.
    CrossRef    

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