Abstract: A screening of fungi capable of degrading dioxins was carried out using the dye Remazol brilliant blue R, as an indicator. Thirty four fungi were found by screening of 255 samples of decayed wood and soil collected from forests. Among them, R-60, R-67 and MG caused a greater decolorization of the dye than any other fungi separated by the screening. Microbial degradation of 2, 8-Dichlorodibenzo-p-Dioxin (2, 8-DCDD) was conducted with these three fungi and with two fungi (PL1 and 267) already screened. The five fungi degraded 50 to 90% of 2, 8-DCDD. Maximum degradation (90%) was obtained with 267 when the incubation was conducted for 30 days after addition of 0.05 mM of 2, 8-DCDD to the culture medium. The rate of degradation rose with the increase in the manganese peroxidase activity in an extracellular crude enzyme solution from each of the five fungi. The levels of enzymatic activity of the three fungi were also similar to those of the two previously screened fungi. The effect of co-cultivation of the screened fungi on degradation of the dioxin was also investigated. However, the degradation was less than that on the cultivation of each fungus alone. Furthermore, 2, 8-DCDD was indeed degraded by the fungi, because 4-Chlorocatechol considered to be an intermediate in the degradation of 2, 8-DCDD was detected among the reaction products.