Abstract: Sixty-five bacterial strains were isolated from urine samples of patients suffering from urinary tract infection and identified by conventional methods. Eighty percent of total isolated organisms were found to be gram negative while remaining 20% were gram positive. Among gram negatives, E. coli and gram positive S. aureus and S. pyogene were the most prevalent organism. The percentage of gram negative isolates was as follows Escherichia coli (47.6%) followed by Pseudomonas aeruginosa (9.2%), Klebsiella pneumoniae (7.6%), Enterobacter aerogenes (6.1%), Proteus mirabilis and Serratia marcescens (4.6% each). The percentage of gram positive isolates includes, Staphylococcus aureus and Streptococcus pyogenes (4.6% each), Enterococcus faecalis, Staphylococcus epidermidis and Bacillus subtilis (3% each) and Staphylococcus saprophyticus (1.5%). The antibiotic resistance of identified organisms was carried out by disc-diffusion method with commercially available disc of fifteen antibiotics having different mode of actions such as cell wall synthesis inhibitors, membrane permeability alternatives, protein synthesis inhibitors and DNA synthesis inhibitors. Gram negatives showed more resistance to these antibiotics as compared to gram positive organisms. The most effective antibiotic for gram negative UTI isolates is gentamycin showing 69.2% efficacy, then sulfamethoxazole-trimethoprim (SXT-TMP) with 55% efficacy and then kanamycin having 50% efficacy. Among gram positives, chloramphenicol is most effective with 84.6% efficacy, then ofloxacin and gentamycin with 76.9% efficacy and then norfloxacin with 69.2% efficacy.