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Pakistan Journal of Biological Sciences

Year: 2004 | Volume: 7 | Issue: 12 | Page No.: 2045-2050
DOI: 10.3923/pjbs.2004.2045.2050
Mitogenic Effect of Bovine β-lactoglobulin and its Proteolytic Digests on Mouse Spleen Resting Cells
R. Mahmud, M. A. Matin and H. Otani

Abstract: Bovine milk β-lactoglobulin was found to induce proliferation of mouse resting spleen cells. Hence, the mitogenic activity was investigated in detail. The cell growth activity was measured by a colorimetric assay using 3-(4, 5-dimethylthiazol-2-yl)-2-5 diphenyl tetrazolium bromide (MTT). A reduced S-carboxymethylated β-lactoglobulin retained mitogenic activity towards mouse spleen cells and the mitogenic activity increased noticeably after digestion with gastrointestinal proteinases, in particular pancreatin. The pancreat n digest also induced proliferation of U266 cells, a human B cell line while it little influenced the growth of a human B cell line, Ball and human T cell lines Jurkat or Molt-3. These results suggest that bovine β-lactoglobulin may involve a mitogenic sequence(s) against some lymphocytes by the action of intestinal proteinases.

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How to cite this article
R. Mahmud, M. A. Matin and H. Otani, 2004. Mitogenic Effect of Bovine β-lactoglobulin and its Proteolytic Digests on Mouse Spleen Resting Cells. Pakistan Journal of Biological Sciences, 7: 2045-2050.

Keywords: mouse spleen cells, mitogenic activity, proteolytic digests and b-lactoglobulin

REFERENCES
  • Goldman, A.S., 2000. Modulation of gastrointestinal tract of infants by human milk: Interface and interactions. An evolutionary perspective. J. Nutr., 130: 4265-4315.
    Direct Link    

  • Lonnerdal, B., 1985. Biochemistry and physiological function of human milk proteins. Am. J. Clin. Nutr., 42: 1299-1317.
    Direct Link    

  • Julius, M.H., M. Janusz and J. Lisowski, 1988. A colostral protein that induce growth and differentiation of resting B-lymphocytes. J. Immunol., 140: 1366-1371.
    Direct Link    

  • Otani, H., M. Monnai and A. Hosono, 1992. Bovine κ-casein as inhibitor of the proliferation of mouse splenocyte induced by lipopolysaccharide stimulation. Milchwissenschaft, 47: 512-515.

  • Pabst, H.F., 1997. Immunomodulation by breast-feeding. Pediatr. Infect. Dis. J., 16: 991-995.
    PubMed    Direct Link    

  • Gill, H.S., F. Doull, K.J. Rutherfurd and M.L. Cross, 2000. Immunoregulatory peptide in bovine milk. Br. J. Nutr., 84: S111-S117.
    Direct Link    

  • Otani, H., Y. Kihara and M. Park, 2000. The immunoenhancing property of dietary casein phosphopeptide preparation in mice. Food Agric. Immunol., 12: 165-173.
    Direct Link    

  • Otani, H., T. Watanabe and Y. Toshiro, 2001. Effect of bovine β-casein fragment 1-28 and its chemically synthesized partial fragments on proliferative response and immunoglobulin production in mouse spleen cell cultures. Biosci. Biotech. Biochem., 65: 2489-2495.
    Direct Link    

  • Otani, H. and M. Monnai, 1993. Inhibition of proliferation of mouse spleen lymphocytes by bovine milk κ-casein digests. Food Agric. Immunol., 5: 219-229.

  • Otani, H., Y. Horimoto and M. Monnai, 1996. Suppression of interleukin-2 receptor expression on mouse CD4+ T cells by bovine κ-caseinoglycopeptide. Biosci. Biotech. Biochem., 60: 1017-1019.
    Direct Link    

  • Otani, H., K. Nakano and T. Kawahara, 2002. Stimulatory effect of dietary casein phosphopeptides preparation on mucosal IgA response of mice to orally ingested lipopolysaccharide from Salmonella typhimurium. Biosci. Biotech. Biochem., 67: 2489-2495.
    Direct Link    

  • Miyauchi, H., I. Shinoda Y. Fukuwatari and H. Hayasawa, 1998. Immunostimulatory effect of bovine lactoferrin pepsin hydrolysate on murine splenocyte and Peyers patch cells. J. Dairy Sci., 80: 2330-2339.

  • Otani, H. and M. Monnai, 1995. Induction of interleukin-1 receptor antagonist-like component produced from mouse spleen cells by bovine κ-caseinoglycopeptide. Biosci. Biotech. Biochem., 59: 1166-1168.
    Direct Link    

  • Matin, M.A., M. Monnai and H. Otani, 2000. Isolation and characterization of a cytotoxic pentapeptide, κ-casecidin, from bovine κ-casein digested with bovine trypsin. Anim. Sci. J., 71: 197-207.
    Direct Link    

  • Kayser, H. and H. Meisel, 1996. Stimulation of human peripheral blood lymphocytes by bioactive peptides derived from bovine milk proteins. FEBS Lett., 383: 18-20.
    CrossRef    PubMed    Direct Link    

  • Hata, I., S. Higashyama and H. Otani, 1998. Identification of a phosphopeptides in bovine casein digest as a factor influencing proliferation and immunoglobulin production in lymphocyte cultures. J. Dairy Res., 65: 569-578.
    Direct Link    

  • Miyauchi, H., M. Hashimoto, I. Nakajima, I. Shinoda Y. Fukuwatari and H. Hayasawa, 1998. Bovine lactoferrin stimulate the phagocytic activity of human neutrophils: Identification of its active domain. Cell. Immunol., 187: 34-37.
    CrossRef    

  • Yoo, Y., R. Watanabe, Y. Koike, M., Mitobe, K. Shimazaki, S. Watanabe and I. Azuma, 1997. Apoptosis in human leukemic cells induced by lactoferricin, a bovine milk protein-derived peptide: Involvement of reactive oxygen species. Biochem. Biophys. Res. Commun., 237: 624-628.
    CrossRef    

  • Susan, G.H., S.M. Alan and S. Lindsay, 1989. β-lactoglobulin. In: Development in Dairy Chemistry-4, Fox, P.F. (Ed.). Elsevier Science Publishers, Ltd., London, pp: 141-153

  • Wong, K.F., N. Midleton, M. Montgomery, M. Dey and R.I. Carr, 1998. Immunostimulation of murine spleen cells by materials associated with bovine milk fractions. J. Dairy Sci., 81: 1825-1832.
    Direct Link    

  • Aschaffenburg, R. and J. Drewry, 1957. Improved method for the preparation of crystalline β-lactoglobulin and α-lactalbumin from cows milk. Biochem. J., 65: 273-277.
    Direct Link    

  • Otani, H. and F. Tokita, 1980. Effects of treatments with urea and some chemical reagents on the antigenicity of β-lactoglobulin. Jap. J. Zootech. Sci., 51: 17-20.

  • Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
    CrossRef    PubMed    Direct Link    

  • Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65: 55-63.
    CrossRef    PubMed    Direct Link    

  • Matin, M.A. and H. Otani, 2002. Cytotoxic and antimicrobial activities of chemically synthesized κ-casecidin and its partial peptides fragmets. J. Dairy Res., 69: 329-334.
    CrossRef    Direct Link    

  • Migliore-Samour, D. and P. Jolles, 1988. Casein, a prohormone with an immunomodulating role for the newborn?. Experimentia, 44: 188-193.
    CrossRef    Direct Link    

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