Abstract: An investigation on prevalence of rice root nematode in the farmer rice fields under natural condition was carried out. Farmer fields were located under Jamalpur district, Tangail district and Mymensingh district. Nematodes were isolated by following Bangladesh plate technique (modified Whitehead and Hemming tray method, 1965) and were counted per liter soil at booting, flowering, milking, soft dough, hard dough and harvesting stage. The highest nematode population 1196.7 L-1 soil was recorded in Madarganj having soil pH 5.9 sandy loam texture, 0.92% OM and higher N status followed by 524.4 L-1 soil in Sutiakhali having soil pH 5.4, loamy texture and 1.06% OM maximum nematode population 965.3 L-1, soil was observed at soft dough stage.