Collection and Grading of Bovine Cumulus-oocyte-complexes (COCs) from Slaughter House Ovaries in View of in vitro Maturation, Fertilization and Culture
Abstract:
The research was carried out at the Animal Breeding and Genetics Laboratory under the Department of Animal Breeding and Genetics, Bangladesh Agricultural University, Mymensingh, Bangladesh. It was conducted with a view to establish some relationship between ovarian condition in terms of the presence or absence of corpus luteum and morphological quality of the COCs as well as the relationship between follicular diameter and quality of COCs. Significantly highest (p<0.01) number of follicles was found in type III ovaries. The number of follicles measuring 2-6 mm diameter was observed to be significantly higher (p<0.01) in type III than type II and type I ovaries. Moreover, grade A and grade B COCs were significantly highest (p<0.01) in number in 2-6 mm diameter follicles. It was established that cumulus cells surrounding the oocytes favour to a less or greater extent the IVM (In vitro maturation), IVF (In vitro fertilization) of oocytes and subsequent IVC (In vitro culture) of zygotes. On the basis of the study, it is concluded that type III ovaries having no corpus luteum may be suggested for obtaining good quality Cumulus-oocyte-complexes (COCs) in experiment for IVM, IVF and subsequent IVC.
How to cite this article
P. C. Goswami, S. Z. Ali, M.A.M. Y. Khandoker, S. A. Azmal, M. K. Alam and R. Khatun, 2004. Collection and Grading of Bovine Cumulus-oocyte-complexes (COCs) from Slaughter House Ovaries in View of in vitro Maturation, Fertilization and Culture. Pakistan Journal of Biological Sciences, 7: 1777-1781.
REFERENCES
Mukherjee, A.B., 1972. Normal progeny from fertilization in vitro of mouse oocytes matured in culture and spermatozoa capacitated in vitro. Nature, 237: 397-398.
CrossRef
Hanada, S., M.L. Saeki and F.S. Choi, 1986. In vitro bovine embryo production. J. Reprod. Fert., 34: 384-390.
Mattioli, M., G. Bacci and E. Seren, 1989. Developmental competence of pig oocytes matured and fertilized in vitro. Theriogenology, 31: 1201-1207.
Direct Link
Fulka, J. Jr. and A. Okolski, 1981. Culture of horse oocytes in vitro. J. Reprod. Fert., 61: 213-215.
PubMed
Alm, H. and H. Torner, 1994. In vitro maturation of horse oocytes. Theriogenology, 42: 345-349.
Zhang, J.J., M.S. Boyle, W.R. Allen and C. Galli, 1989. Recent studies on in vivo fertilization of in vitro matured horse oocytes. Equine Vet. J., 21: 101-104.
CrossRef
Hinrich, K., D.F. Kenney and R.M. Kenney, 1990. Aspiration of oocytes from mature and immature preovulatory follicles in the mare. Theriogenology, 34: 107-112.
PubMed
Bavister, B.D., T.A. Rose-Hellekant and T. Pinyopummintr, 1992. Development of in vitro matured/in vitro fertilized bovine embryos into morulae and blastocysts in defined culture media. Theriogenology, 37: 127-146.
CrossRef Direct Link
Fukui, Y. and H. Ono, 1989. Effect of sera, hormones and granulosa cells added to culture media for in vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fert., 86: 501-506.
Direct Link
Tsafri, A. and C.P. Channing, 1975. Influence of folicular maturation and culture conditions on the meiosis of pig oocytes in vitro. J. Reprod. Fert., 43: 149-152.
CrossRef
Thibault, C., 1977. Are follicular maturation and oocyte maturation independent processes? J. Reprod. Fert., 51: 1-15.
PubMed
Fukushima, M. and Y. Fukui, 1985. Effect of gonadotropins and steroids on the subsequent fertilizability of extrafollicular bovine oocytes cultured in vitro. Anim. Reprod. Sci., 9: 323-332.
Direct Link
Moor, R. M., C. Polge and S.M. Wiladsen, 1980. Effect of follicular steroids on the maturation and fertilization of mammalian oocytes. J. Embryol. Exp. Morphol., 56: 319-335.
Direct Link
Crister, E.S., M.L. Leibfried-Rutledge and N.L. First, 1986. Influence of cumulus cell association during in vitro maturation of bovine oocytes on embryonic development. Biol. Reprod., 34: 192-192.
Steel, R.G.D. and J.H. Torrie, 1980. Principles and Procedures of Statistics. 2nd Edn., McGraw Hill Co., New York
Snedecor, G.W. and W.G. Cochran, 1980. Statistical Methods. 7th Edn., Iowa State University Press, Ames, USA., pp: 271-273
Webb, R., B.K. Campbell, H.A. Garveric and J.G. Gong, 1999. Molecular mechanisms regulating follicular recruitment and selection. J. Reprod. Fert., 54: 33-48.
PubMed
Savio, J.D., L. Keenan, M.P. Boland and J.F. Roche, 1988. Pattern of growth of dominant follicles during the estrous cycle of heifers. J. Reprod. Fert., 83: 663-671.
CrossRef
Fortune, J.E., 1994. Ovarian follicular growth and development in mammals. Biol. Reprod., 50: 225-232.
CrossRef PubMed Direct Link
Ginther, O.J., M.C. Wiltbank, P.M. Fricke, J.R. Gibbons and K. Kot, 1996. Selection of dominant follicles in cattle. Biol. Reprod., 55: 1187-1194.
PubMed
Hafez, E.S.E., 1993. Reproduction in Farm Animals. 6th Edn., Lea and Febiger, Philadelphia, pp: 69-143
Totey, S.M., C.H. Pawshe and G.P. Singh, 1993. In vitro maturartiom and fertilization of buffalo oocytes (Bubalus bubalis): Effects of media, hormones and sera. Theriogenology, 39: 1153-1171.
PubMed
Rath, D., H. Niemann and T. Tao, 1995. In vitro maturation of porcine oocytes in follicular fluid with subsequent effects on fertilization and embryo yield in vitro. Thriogenology, 44: 529-538.
Direct Link
Hashimoto, S., K. Saeki, Y. Nagano, N. Minami, M. Uamada and K. Utsumi, 1998. Effect of cumulus density during in vitro maturation on the developmental competence of bovine oocytes. Theriogenology, 49: 1451-1463.
Direct Link
Yamauchi, N., Y. Fukui and T. Nagai, 1999. Male pronucleus formation in denuded porcine oocytes after in vitro maturation in the presence of cysteamine. Biol. Reprod., 61: 828-833.
PubMed
© Science Alert. All Rights Reserved