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Pakistan Journal of Biological Sciences

Year: 2004 | Volume: 7 | Issue: 10 | Page No.: 1676-1683
DOI: 10.3923/pjbs.2004.1676.1683
Development of a Second Generation Genetic Linkage Map for Sour Cherry Using SSR Markers
Fatih A. Canli

Abstract: A second generation consensus linkage map of two tetraploid sour cherry cultivars (Prunus cerasus L., 2n=4x=32), ‘Rheinische Schattenmorelle’ (RS) and ‘Erdi Botermo’ (EB) was constructed. Forty five SSR primer pairs from apple, peach, sour cherry and sweet cherry were screened and 10 informative SSRs yielding 17 markers were added to the sour cherry linkage map. The EB and RS consensus map consisted of 161 markers covering 442.4 cM in 19 linkage groups. The largest linkage group is group 9 covering 44.5 cM. The average distance between two markers was 2.74 cM. The longest distance between two adjacent markers was 16.9 cM in linkage group 8. SSR markers tightly linked to important quantitative traits in sour cherry, such as bloom time, fruit weight and pistil death, were obtained. These markers could be utilized as a valuable tool for trait selection. The results from current study have shown that SSR primers are co-dominant, reproducible, highly polymorphic and have high utility for cross-species amplification within Prunus. SSR will be the marker of choice for comparative mapping and MAS studies in Prunus.

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How to cite this article
Fatih A. Canli , 2004. Development of a Second Generation Genetic Linkage Map for Sour Cherry Using SSR Markers. Pakistan Journal of Biological Sciences, 7: 1676-1683.

Keywords: sour cherry boom time, mas, qtls, linkage mapping ssrs and prunus

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