Isolation, Purification, Characterization of Cellulolytic Enzymes Produced
by the Isolate Streptomyces omiyaensis
Abstract:
A Streptomyces strain A2 which was provisionally identified
as Streptomyces omiyaensis, isolated from goat`s rumen was found capable
of producing cellulolytic enzymes during growth on different cellulosic substrates.
The isolate A2 grown under different conditions showed that CMC was
the best cellulosic substrates for inducing the synthesis of extracellular cellulolytic
enzymes. The isolate also showed heavy growth and liquefaction at pH 6.5, temperature
35 to 40°C and 5 days of incubation period during growth in liquid
Winsted`s media having 1.2% CMC as a cellulose substrate. Maximum level of CMC-ase
(230.56 U mL-1) in liquid media was found to produce when beef extract
used as a nitrogen source along with CMC as cellulose source. Maximum level of
reducing sugar (473.33 μg mL-1) and protein (324.27 μg mL-1)
was obtained when CMC used as a growth substrate. The crude enzyme of the isolate
was found to show highest enzyme activity (CMC-ase of 269.44 U mL-1,
FP-ase of 64.81 U mL-1, Avicelase of 200 U mL-1 and β-Glucosidase
of 138.89 U mL-1) at pH 6.5 and temperature 45°C during enzyme substrate
reaction. The molecular weight of enzyme of the isolate Streptomyces
omiyaensis was determined by SDS-PAGE technique and found 85 kDa.
How to cite this article
M. Z. Alam, M. A. Manchur and M. N. Anwar, 2004. Isolation, Purification, Characterization of Cellulolytic Enzymes Produced
by the Isolate Streptomyces omiyaensis. Pakistan Journal of Biological Sciences, 7: 1647-1653.
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