Production and Some Properties of Protease Produced by Bacillus licheniformis
Isolated from Tihamet Aseer, Saudi Arabia
Abstract:
Culture conditions affecting protease production by Bacillus licheniformis
isolated from Tihamet Aseer, Saudi Arabia were investigated. The bacterium strain
gave a maximum protease production of 221 U mL-1 when growing in a
casein broth medium after 36 h compared with gelatin broth medium. Optimum pH
and temperature of protease production were 8 and 50°C, respectively. The highest
level of protease production in the presence of soybean meal as a carbon source
and the peptone as a nitrogen source was obtained. The protease was optimally
active at pH 9 and 55°C . The enzyme was stable at temperature range of 60-65°C
during the period tested (1h) and retaining more than 85% of its activity at 70°C.
These properties make the enzyme suitable for detergent industry.
How to cite this article
Al- Shehri, M. Abdulrahman and Mostafa S. Yasser, 2004. Production and Some Properties of Protease Produced by Bacillus licheniformis
Isolated from Tihamet Aseer, Saudi Arabia. Pakistan Journal of Biological Sciences, 7: 1631-1635.
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