Abstract: In this study, the influence of various concentrations of wastewater medium, nitrogen, phosphorus, potassium and soda ash (Na2CO3) using batch cultivation for S. platensis growth were examined. It was found that modified wastewater medium, which contains a 1:11 dilution ratio of wastewater, supplemented with 0.09 g L-1 nitrate, 0.59 g L-1 phosphate, 0.18 g L-1 potassium and 3 g L-1 Na2CO3, shows great potential for cultivating S. platensis. The growth of the algae was smooth in the modified culture. Apart from chlorophyll (2.36 mg g-1), approximately 1.0 g L-1 biomass, 59% protein and 14% phycocyanin were detected which was almost identical to that of Zarrouk medium.