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Pakistan Journal of Biological Sciences

Year: 2004 | Volume: 7 | Issue: 8 | Page No.: 1443-1452
DOI: 10.3923/pjbs.2004.1443.1452
Morphogenetic Effect of Growth Hormones i.e., Indole-3-Acetic Acid, Gibberellic Acid and Heavy Metal i.e., Lead Nitrate on the External and Internal Morphology of Seedlings of Cicer arietinum L.
Tahseen Fatima and N. Y. Chaudhry

Abstract: The present study on Cicer arietinum L., deals with the morphogenetic effects of growth hormones i.e., IAA, GA3 and heavy metal i.e., Pb(NO3)2 on the external and internal morphology of the plant. Following concentrations of hormones and heavy metal were used individually; 200 ppm IAA, 200 ppm GA3 and 50 ppm Pb(NO3)2. In combination 200 ppm IAA+200 ppm GA3, 200 ppm IAA+50 ppm Pb(NO3)2, 200 ppm GA3+50 ppm Pb(NO3)2, 200 ppm IAA+200 ppm GA3+50 ppm Pb(NO3)2. Results were collected after 15 days and compared with control. Extraneous IAA inhibited elongation in root, hypocotyl and shoot, which was accompanied by increase in diameter. Contrarily, increase in length accompanied by inhibited diameter was observed with the doses of GA3. Applied 50 ppm Pb(NO3)2 individually as well as in combination with GA3 and in the mixture of all three doses inhibited length. Number of rootlets increased with the application of IAA and were inhibited with Pb(NO3)2. Branching was promoted by IAA and IAA+GA3. Number of compound leaves increased with IAA, IAA+GA3 treatment. Moreover number of leaflets in first three compound leaves decreased with Pb(NO3)2 and IAA+Pb(NO3)2. Leaflet area decreased with Pb(NO3)2 and GA3+Pb(NO3)2 treatment. In the internal morphology of root, shoot and rachis epidermal cells remained more or less constant in treated plants. Width of cortical region, cortical cells, xylary region and pith of root and shoot showed expansion with IAA and inhibition with GA3 applications. 200 ppm IAA showed expansion in width and early maturation of metaxylem elements. Width of rachis and ground tissue showed inhibition with GA3, Pb(NO3)2 and GA3+Pb(NO3)2 applications. Number of vascular bundles of rachis remained same except with Pb(NO3)2 and GA3+Pb(NO3)2 which showed inhibition in number.

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How to cite this article
Tahseen Fatima and N. Y. Chaudhry, 2004. Morphogenetic Effect of Growth Hormones i.e., Indole-3-Acetic Acid, Gibberellic Acid and Heavy Metal i.e., Lead Nitrate on the External and Internal Morphology of Seedlings of Cicer arietinum L.. Pakistan Journal of Biological Sciences, 7: 1443-1452.

Keywords: cicer arietinum l root shoot, leaf cortex, xylary region, hormones, iaa, ga and heavy metal pb no

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