Abstract: This study was conducted to obtain virus-free propagation materials from Granola, Pasinler 92 and Caspar potato (Solanum tuberosum L.) cultivars infected with potato virus X (PVX), potato virus Y (PVY) and potato leaf roll virus (PLRV) by using meristem-tip culture. For in vitro propagation, it was tested the effect of different combinations and concentrations of benzylamino purine (BA) (0.0, 0.25, 0.50 mg L-1) and gibberellic acid (GA3) (0.0, 0.25, 0.50 mg L-1) on the number of shoot and node. On the other hand, it was evaluated the effect of BA (0.00, 5.00, 10.0 mg L-1) and CCC (chlorocholine chloride) (0.00, 500 mg L-1) on the tuberization under two photoperiodic regimes (light and dark). The MS salts and vitamins supplemented with 30 g L-1 sucrose was used as a medium and the media was solidified with 7.0 g L-1 agar and the ratio of sucrose added into media for micro tuber production had been increased from 3-8%. The highest number of shoots was obtained from 0.00/0.25, 0.25/0.50 and 0.00/0.00 mg L-1 BA/GA3 treatments for Granola, Pasinler 92 and Caspar cultivars as 1.52, 1.24 and 1.44, respectively. However, the highest number of node were determined on 0.00/0.50 for Granola (9.12), Pasinler 92 (8.76) and on 0.00/0.25 mg L-1 BA/GA3 treatments for Caspar (8.24). When the results were assayed according to total tuber number, the most micro-tubers for Granola, Pasinler 92 and Caspar cultivars were obtained from 5.00/5000 mg L-1 BA/CCC treatment as 5.6, 4.0, 4.8 per/bottle under dark treatments. All in vitro regenerated plant materials were tested by DAS-ELISA (double antibody sandwich enzyme-linked immunosorbent assay) to determine the presence and absence of viruses and PVX, PVY and PLRV viruses were eliminated from Granola (25, 40 and 60%), Pasinler 92 (16, 41.6 and 46.1%) and Caspar cultivars (28.5, 33.3 and 50%), respectively.