HOME JOURNALS CONTACT

Pakistan Journal of Biological Sciences

Year: 2002 | Volume: 5 | Issue: 8 | Page No.: 871-877
DOI: 10.3923/pjbs.2002.871.877
Molecular Cloning and Sequencing of D-mandelate Dehydrogenase Gene from Rhodotorula graminis
Rosli Md.Illias, Graem A. Reid, Stephen K. Chapman, Charles A. Fewson and John S. Miles

Abstract: The yeast Rhodotorula graminis can use D, L-mandelate as a source of carbon and energy. We have isolated the gene encoding D-mandelate dehydrogenase, one of the two enzymes that stereospecifically catalyze the first step in mandelate degradation. The sequences of the genomic DNA and a cDNA prepared by RT-PCR revealed the presence of three short introns within the coding region. The predicted amino acid sequence of D-mandelate dehydrogenase is 27-33% identical to other members of a large family of NAD+-dependent 2-hydroacid dehydrogenase from a broad spectrum of bacteria and eukaryotes and it has a wide range of substrate specificities.

Fulltext PDF

How to cite this article
Rosli Md.Illias, Graem A. Reid, Stephen K. Chapman, Charles A. Fewson and John S. Miles, 2002. Molecular Cloning and Sequencing of D-mandelate Dehydrogenase Gene from Rhodotorula graminis. Pakistan Journal of Biological Sciences, 5: 871-877.

Keywords: Mandelate dehydrogenase, rhodotorula, gene, cloning, sequencing, yeast and bacteris

REFERENCES
  • Baker, D.P. and C.A. Fewson, 1989. Purification and characterization of D (-)-mandelate dehydrogenase from Rhodotorula graminis. J. Gen. Microbiol., 135: 2305-2344.

  • Basak, A.K., D.P. Baker, C.A. Fewson and D.I. Stuart, 1993. Preliminary crystallographic study of D mandelate dehydrogenase from Rhodotorula graminis. J. Mol. Biol., 233: 781-783.

  • Crowl, R., C. Seamans, P. Lomedico and S. McAndrew, 1985. Versatile expression vectors for high-level synthesis of cloned gene products in Escherichia coli. Genetics, 38: 31-38.

  • Durham, D.R., 1984. Initial reactions involved in the dissimilation of mandelate by Rhodotorula graminis. J. Bacteriol., 160: 778-780.

  • Feinberg, A.P. and B. Vogelstein, 1983. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem., 132: 6-13.
    CrossRef    Direct Link    

  • Fewson, C.A., 1988. Microbial metabolism of mandelate: A microcosm of diversity. FEMS Microbiol. Rev., 54: 85-110.

  • Fewson, C.A., 1992. Microbial Metabolism and Mandelate: Occurrence, Properties and Evolution of Mandelate Dehydrogenase and other Enzymes of the Mandelate Pathway. In: The Evolution of Metabolic Function, Mortlock, R.P. (Ed.). CRC Press, Boca Raton, pp: 115-141

  • Vaslet, A.L., A. Sykes and R.L. Strausberg, 1988. Nucleotide sequence of gene for phenylalanine ammonia-lyase from Rhodotorula rubra. Nucl. Acid Res., 16: 11381-11381.

  • Goldberg, J.D., T. Yoshida and P. Brick, 1994. Crystal structure of a NAD-dependent D-glycerate dehydrogenase at 2.4 Å resolutions. J. Mol. Biol., 236: 1123-1140.

  • Haid, A. and M. Suissa, 1984. Immunochemical detection of membrane proteins after sodium dodecyl sulfate polyacrylamide gel electrophoresis. Methematics Enzymol., 96: 192-205.

  • Hosono, K., S. Kajiwara, Y. Yamazaki and H. Maeda, 1990. Construction of a bioreactor for production of (R)-(-)-mandelate, a typical specialty chemical. J. Biotechnol., 14: 149-156.

  • Illias, R.M., R. Sinclair, D. Robertson, A. Neu, S.K. Chapman and G.A. Reid, 1998. L-mandelate dehydrogenase from Rhodotorula graminis: Cloning, sequence and kinetic characterization of the recombinant enzyme and its independently expressed flavin domain. Biochem. J., 333: 107-115.

  • Kochhar, S., N. Chuard and H. Hottinger, 1992. Glutamate 264 modulates the pH dependence of NAD-dependent D-lactate + dehydrogenase. J. Biol. Chem., 267: 20298-20301.

  • Kochhar, S., P.E. Hunziker, P. Leong-Morgenthaler and H. Hottinger, 1992. Primary structure, physicochemical properties and chemical modification of NAD-dependent D-lactate dehydrogenase. J. Biol. Chem., 267: 8499-8513.

  • Lamzin, V.S., Z. Dauter, V.O. Popov, E.H. Harutyunyan and K.S. Wilson, 1994. High resolution structures of holo and apo-formate dehydrogenase. J. Mol. Biol., 236: 759-785.

  • Orbach, M.J., E.B. Porro and C. Yanovsky, 1986. Cloning and Characterization of the gene B-tubulin and its use as a dominant selectable marker. Mol. Cell. Biol., 6: 2452-2461.
    Direct Link    

  • Popov, V.O. and V.S. Lamzin, 1994. NAD dependent formate + dehydrogenase. Biochem. J., 301: 652-663.

  • Rasmussen, O.F. and H. Orum, 1991. Analysis of the gene for phenylalanine ammonia-lyase from Rhodosporidium toruloides. DNA Seq., 1: 207-211.

  • Rokeach, L.A., J.A. Haselby and S.O. Hoch, 1988. Molecular cloning of a cDNA encoding the human SM-D autoantigen. Proc. Nat. Acad. Sci. USA., 85: 4832-4836.

  • Ruby, S.W. and J. Abelson, 1991. Pre-mRNA splicing in yeast. Trend. Gen., 7: 79-85.

  • Sambrook, J., E.F. Fritsch and T.A. Maniatis, 1989. Molecular Cloning: A Laboratory Manual. 2nd Edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, USA., ISBN-13: 9780879695774, Pages: 397
    Direct Link    

  • Sanger, F., S. Nicklen and A.R. Coulson, 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A., 74: 5463-5467.
    CrossRef    PubMed    Direct Link    

  • Stanley, K.K. and J.P. Luzio, 1984. Construction of a new family of high efficiency bacterial vectors: Identification of cDNA clones coding for human liver proteins. EMBO J., 3: 1429-1434.

  • Vasik-Racki, D., M. Jonas, C. Wandrey, W. Hummel and M.R. Kula, 1989. Continuous (R)-mandelic acid production in an enzyme membrane reactor. Applied Microbiol. Biotechnol., 31: 215-222.

  • Viera, J. and J. Messing, 1987. Production of single stranded plasmid DNA. Methematics Enzymol., 153: 3-11.

  • Yamazaki, Y. and H. Maeda, 1986. Enzymatic synthesis of optically pure (R)-(-)-mandelic acid and other 2-hydroxy carboxylic acids- screening for the enzyme and its purification, characterization and use. Agric. Biol. Chem., 20: 2621-2631.

  • Yasin, M. and C.A. Fewson, 1993. L. Mandelate dehydrogenase from Rhodotorula graminis: Purification, partial characterization and identification as a flavocytochrome b. Biochem. J., 293: 455-460.

  • Yoshida, T., K. Yamaguchi, T. Hagishita, T. Mitsunaga and A. Miyata et al., 1994. Cloning and expression of the gene for hydroxypyruvate reductase (D-lycerate dehydrogenase) from an obligate methylotroph Hyphomicrobium methylovorum GM2. Eur. J. Biochem., 223: 727-732.

    • © Science Alert. All Rights Reserved