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Pakistan Journal of Biological Sciences

Year: 2002 | Volume: 5 | Issue: 8 | Page No.: 869-870
DOI: 10.3923/pjbs.2002.869.870
An Improved Genomic In situ Hybridization (GISH) Method to Differentiate Chromosomes from Closely Related Genomes
N. Iqbal, S. M. Reader, P.D. S. Caligari and T. E. Miller

Abstract: Previously reported genomic in situ hybridization protocols failed to differentiate Aegilops uniaristata chromosomes in a polyploid wheat background. Different protocols and hybridization temperatures were tested to optimize the differentiation of N genome A. uniaristata chromosomes from those of the A, B and D genomes of wheat. A combination of pre-hybridization of cytological preparations, pre-annealing of the genomic probe with blocking DNA and hybridization at elevated temperatures proved successful. A genomic in situ hybridization protocol for the differentiation of chromosomes from very closely related genomes in a polyploid background is reported.

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How to cite this article
N. Iqbal, S. M. Reader, P.D. S. Caligari and T. E. Miller, 2002. An Improved Genomic In situ Hybridization (GISH) Method to Differentiate Chromosomes from Closely Related Genomes. Pakistan Journal of Biological Sciences, 5: 869-870.

Keywords: Genomic in situ hybridization, GISH, A, uniaristata, wheat and addition lines

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