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Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 6 | Page No.: 670-671
DOI: 10.3923/pjbs.2001.670.671
Effect of Gamma Irradiation on the Callus, Developed from Indica Rice
M. F. Hossain and M. S. Alam

Abstract: Induced mutation holds promise for effective improvement and have high potential for bringing about genetic variability. Regenerable calli of four rice varieties were exposed to 0 to 6 Gy of gamma rays to determine their effect on growth and plant regeneration capability. Both growth and regeneration capacity decreased with increasing levels of gamma rays ; however, plant regeneration capacity was more sensitive to gamma rays than growth. The 50% inhibition dose for callus growth and plant regeneration was approximately 5.0 Gy of gamma radiation in Binnatoa variety. But in remaining varieties 4.0 Gy was recorded for callus growth and 2.0 Gy for regeneration.

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How to cite this article
M. F. Hossain and M. S. Alam, 2001. Effect of Gamma Irradiation on the Callus, Developed from Indica Rice. Pakistan Journal of Biological Sciences, 4: 670-671.

Keywords: Indica rice, gamma radiation, callus growth and plant regeneration

REFERENCES

  • Gao, M.W., Q.H. Cai and Q. Liang, 1992. In vitro culture of hybrid indica rice combined with mutagenesis. Plant Breed., 108: 104-110.
    CrossRef    Direct Link    


  • Khanduja, S.D. and V.L. Goel, 1986. Pattern of variability in some fuel wood trees grown on sodic soils. Indian For., 112: 118-123.


  • Lu, Y.M., C.L. Wang, M. Shen and Q.F. Chen, 1997. Efect of gamma irradiation on the formation of calli and regeneration of green plants in rice anther culture. Acta Agric. Scand., 9: 123-126.
    Direct Link    


  • Min, S., Z. Xiang, X. Qi and C. Zhao, 1991. Effects of gamma-ray radiation treatment on somatic cell cultura in rice (Oryza sativa L.). Cer. Res. Comm., 19: 201-208.


  • Murashige, T. and F. Skoog, 1962. A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant., 15: 473-497.
    CrossRef    Direct Link    


  • Pongtongkam, P., P. Ratisoontorn, S. Suputitada, S. Peyachoknagul and L. Ngernsiri et al., 1994. Induced mutation in rice by tissue culture. Kasetsat J. Nat. Sci., 28: 381-389.


  • Sakila, M., S.M. Ibrahim and A. Kalamani, 1999. In vitro mutagenesis in scented rice cultivars. Rice Biotecnol. Q., 38: 30-34.


  • Zapata F.J., R.R. Aldemita, L.B. Torrizo, A.U. Novero, S.K. Rina and R.R. Rola, 1986. Anther culture of Basmati 370 at IRRI: A Gamma-ray induced green plant regeneration. IRRN., 2: 22-23.


  • Zhang, J.J., K.H. Fan and Z.H. Zhang, 1994. Study on variation in rice (Oryza sativa L.). Somaclones from in vitro irradiated somatocytes. Acta Agric. Shanghai, 10: 6-11.

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