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Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 10 | Page No.: 1291-1293
DOI: 10.3923/pjbs.2001.1291.1293
The Effect of Two Extracts from Stichopus badionotus Selenka upon Induced Pleurisy in Rat
S. Z. Idid, D. M. Jalaluddin, B. H. Ridzwan, A. Bukhori, S. Nor Hazlinah, C. C. Hoo and L. K. Marthivarman

Abstract: The pleurisy model in rats was used to study the pharmacology of extracts from the sea-cucumber Stichopus badionotus Selenka. Ten control animals were injected intrapleurally with 0.1ml carageenan (1%) and their pleural cavities were examined. Three groups (six animals per group) were treated with concentrations of 5, 10 and 20 mg/ml of the ethanol extract of sea-cucumber given i.p. and three other groups were treated with similar concentrations of a phosphate-buffer extract also given i.p. A half-hour later the animals were administered carageenan (i.p.). These animals were killed four-hours after the administration of carageenan and their pleural cavities were examined. The results showed that the extracts were able to reduce pleural effusion, neutrophil counts, and also adenosine deaminase activity significantly and in a dose-dependent manner. The effects were more pronounced with ethanol extract compared to the phosphate-buffer extract.

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How to cite this article
S. Z. Idid, D. M. Jalaluddin, B. H. Ridzwan, A. Bukhori, S. Nor Hazlinah, C. C. Hoo and L. K. Marthivarman, 2001. The Effect of Two Extracts from Stichopus badionotus Selenka upon Induced Pleurisy in Rat. Pakistan Journal of Biological Sciences, 4: 1291-1293.

Keywords: pleural effusion, carageenan, pleurisy and sea-cucumber

REFERENCES
  • Anisimov, M.M., N.G. Prokofieva, L.Y. Korotkikh, I.I. Kapustina and V.A. Stonik, 1980. Comparative study of cytotoxic activity of triterpene glycosides from marine organisms. Toxiconolgy, 18: 221-223.

  • Bullivant, M.L. and I.G. Otterness, 1988. Carageenan pleurisy. Methods Enzymol., 62: 334-338.

  • Cronstein, B.N., D. Naime and E. Ostad, 1993. The anti inflammatory mechanism of methotrexate: Increased adenosine release at inflamed sites diminishes leucocyte accumulation in an In vivo model of inflammation. J. Clin. Invest., 92: 2675-2682.

  • Galanti, B., S. Nordiello, M. Russo and F. Florentino, 1981. Increased lymphocyte adenosine deaminase in typhoid fever. Scand. J. Infect. Dis., 13: 47-50.

  • Hassan, Y., N. Yeap, K.H. Kim and M.M. Shahimi, 1996. Effects of sea-cucumber water extract on systemic anaphylactic reaction. Malaysian J. Med. Sci. (Suppl)., 3: 37-38.

  • Kanagawa, H., H. Fujiwara, S. Shoji, T. Fujii and S. Kudoh et al., 1997. Adenosine modulates endothelin-induced brobchoconstriction in guinea-pig airway. Int. Arch. Allergy Immunol., 112: 83-87.

  • Kaswandi, M.A., B. Safran, M.B.O. Rozali and B.H. Ridzwan, 1993. Kesan-kesan fisiologi ekstrak kasar saponin daripada empat spesies timun-laut. Proc. Simp. Sumber Alam Trop. UNIMAS, 6: 11-11.

  • Kuznetsova, T.A., M.M. Anisimov, A.M. Popov, S.I. Baranova and S. Afiyatullov et al., 1982. A comparative study in vitro of physiological activity of triterpene glycosides of marine invertebrates of echinoderm type. Comp. Physiol., 73: 41-43.

  • Olah, M.E. and G.I. Stiles, 1995. Adenosine receptor subtypes: Characterization and therapeutic regulation. Ann. Rev. Pharmacol. Toxicol., 35: 581-606.

  • Ridzwan, B.H., M.A. Kaswandi, Y. Azman and M. Fuad, 1995. Screening for antibacterial agents in three species of sea cucumber from coastal areas of Sabah. Gen. Pharmacol., 26: 1539-1543.
    CrossRef    PubMed    Direct Link    

  • Saleh, T.S.F., J.B. Calixto and Y.S. Medeiros, 1999. Effects of anti-inflammatory drugs upon nitrate and myeloperoxidase levels in the mouse pleurisy induced by carageenan. Peptides, 20: 949-956.

  • Shimada, S., 1969. Antifungal steroid glycoside from sea-cucumber. Sci., 163: 1462-1471.

  • Thomas, C.E. and D.J. Reed, 1989. Current status of calcium in hepatocellular injury. Hepatology, 10: 375-384.

  • Van Der Velden, V.H.J., 1998. Glucocorticoids: Mechanism of action and anti-inflammatory potential in asthma. Mediators Inflamm., 7: 229-237.

  • Van der Weyden, M.B. and W.N. Kelly, 1976. Human adenosine deaminase distribution and properties. J. Biol. Chem., 251: 5448-5456.

  • Vinegar, R., J.F. Truax, J.L. Selph and F.A. Voelker, 1982. Pathway of onset, development and decay of carrageenan pleurisy in the rat. Fed. Proc., 41: 2588-2595.
    PubMed    

  • Yasumoto, T., K. Nakamura and Y. Hashimoto, 1967. A new saponin, holothurin B, isolated from sea-cucumber Holothuria vagabondaand Holothuria lubrica. Agric. Biol. Chem., 31: 7-10.

  • Zainuddin, M., Y.T. Chua, S.Z. Idid and M. Paden, 1986. Air gamat (sea-cucumber (holothurian extract) does not have β-adrenoceptor agonist or phosphodiesterase inhibiting properties. Perubatan UKM, 8: 41-48.

  • Davies, H., 1997. Introductory Immunology. Chapman and Hall, London

  • Giusti, G., 1974. Adenosine Deaminase. In: Methods of Enzymatic Analysis, Bergmeyer, H.U. (Ed.). Academic Press, New York, pp: 1092-1096

  • Ridzwan, B.H., B.I. Nor, B.I. Norhayati, M.A. Kaswandi and M.B.O. Rozali, 1990. Timun laut (holothuroid) Sabah: Jus Kasar beberapa spesies sebagai penyembuh luka aruhan. Proceedings of the 7th National Seminar on Natural Products, (NSNP`90), USM and Institut Kimia Malaysia (Utara), pp: 204-209.

  • Bradford, M.M., 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72: 248-254.
    CrossRef    PubMed    Direct Link    

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