HOME JOURNALS CONTACT

Pakistan Journal of Biological Sciences

Year: 2001 | Volume: 4 | Issue: 10 | Page No.: 1267-1270
DOI: 10.3923/pjbs.2001.1267.1270
Effect of Pyrolidine and its Copper Complex on Rabbit Tyrosinase
Galila A. Yacout

Abstract: The effect of pyrolidine (L) or its copper-complex © on rabbit melanocytes tyrosinase were studied. The results revealed that (L) appeared to be a non competitive inhibitor of tyrosinase for about 30% , while ( C ) was an activator for about 4.6 fold. In addition, general effects of (L) or ( C ) on rabbit were studied via serum parameters. The obtained data afforded an additional biological effect of the tested compounds .

Fulltext PDF

How to cite this article
Galila A. Yacout , 2001. Effect of Pyrolidine and its Copper Complex on Rabbit Tyrosinase. Pakistan Journal of Biological Sciences, 4: 1267-1270.

Keywords: Tyrosinase, pyrolidine, copper-complex, inhibiton and activation

REFERENCES
  • Agrup, P., R. Carstam, A. Wittbjer, H. Rosman and E. Rosengren, 1989. Tyrosinase activity in serum from patients with malignant melanoma. Acta Derm. Venerol., 69: 120-124.

  • Burnett, J., 1971. The tyrosinase of mouse melanoma. J. Biochem., 246: B3079-B3091.

  • Cory, J. and E. Frieden, 1967. Differential reactivities of tyrosine residues of proteins to tyrosinase. Biochemistry, 6: 121-126.

  • Dawson, R., G. Milne and R. Williams, 1982. Changes in the collagen of rat heart in copper deficiency-induced cardiac hypertrophy. Cardiovas. Res., 16: 559-565.

  • Duckworth, H.W. and J.E. Coleman, 1970. Physicochemical and kinetic properties of mushroom tyrosinase. J. Biol. Chem., 245: 1613-1625.
    Direct Link    

  • Hearing, V., T. Ekel, P. Montague, E. Hearing and J. Nicholson, 1978. Mammalian tyrosinase: Isolation by simple new procedure and characterization of its steric requirements for cofactor activity. Arch. Biochem. Biophys., 185: 407-418.

  • Jolley, R., D. Robb and H. Mason, 1969. The multiple forms of mushroom tyrosinase: Association B Dissociation phenomena. J. Bio. Chem., 244: 1593-1599.

  • Jolley, R., L. Evans, N. Makino and H. Mason, 1974. Oxytyrosinase. J. Biochem., 249: 335-339.

  • Katoch, R., G. Trivedi and R. Phadke, 1999. 1-[2-hydroxy-3-octadecan-1-oate]propyl-tetramethyl pyrolidine-N-oxyl-3-carboxylate as a potential spin probe for membrane structure studies. Bioorg. Med. Chem., 7: 2753-2758.

  • Kovacs, S., K. Geekie, R. Oxenhandler and P. Agris, 1981. Tyrosinase activity and isoenzyme distribution corresponding to growth and regression of melanoma in sinclair miniature swine. J. Natl. Cancer Inst., 67: 645B-651B.

  • Lehninger, A., D. Nelson and M. Cox, 1993. Principles of Biochemistry. 2nd Edn., Worth Publishers, USA., Pages: 674

  • Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
    CrossRef    PubMed    Direct Link    

  • Sethy, V., J. Francis, R. Russel and P. Ruppel, 1988. Dual effect of N-methyl-N-(1-methyl-4-pyrolidino)-2-butyl)acetamide on release of (3H)-acetyl choline from the rat hippocampal slices. Neuropharmacol., 27: 1191B-1195B.

  • Sukhanov, V., V. Diakov, V. Lalaev and A. Lakhiaev, 1991. Heterogeneity of tyrosinases isolated from rat skin, normal human skin and human melanoma tumors. Biokhimia, 56: 1026B-1035B.

  • Strothkamp, K. and H. Mason, 1974. Pseudoperoxidase activity of mushroom tyrosinase. Biochem. Biophys. Res. Comm., 61: 827-832.

  • Wallach, M., 1999. Interpretation of Diagnostic Tests. 7th Edn., Wolters Kluwer Company, London, pp: 35-44

  • Lineweaver, H. and D. Burk, 1934. The determination of enzyme dissociation constants. J. Am. Chem. Soc., 56: 658-666.
    CrossRef    Direct Link    

    • © Science Alert. All Rights Reserved