Abstract:
The recovery of both mineralized and applied N in soil-plant system is low throughout the world. Leaching, denitrification and NH3 volatilization are thought to be the major N loss mechanisms responsible for low N recovery. The aim of this study was to evaluate the effect of nitrification inhibitor nitrapyrin on N transformations, recovery and to prevent formation of
Introduction
The yield potential of crops and productivity of forage species are often constrained by the supply of nitrogen (N). To overcome this limitation, N fertilizers are commonly applied to grasses, important food and cash crops. It has been suggested (Garret, 1993) that at least half the productivity of modern temperate grassland systems is due to the fertilizer N input. Supply of fertilizer N not only increases primary plant and subsequent animal production, but also allowed farmers a good deal of flexibility in the management of their enterprises. According to Munro and Davies (1973), in all temperate grassland regions N supply and pasture production are closely linked and yield is generally proportional to the supply of fertilizer N.
Nitrogen fertilizer is an expensive input and in many trials in Australia less than 50 percent of applied N is recovered in the crop and soil with the remainder being lost (Freney et al., 1993; Rochester et al., 1996). The apparent recovery of N applied to grass grown in the United Kingdom varies widely, but a value in the range of 50-70 percent is frequently observed (Morrison et al., 1980). A recovery of less than 45 percent from a single application of N fertilizer was reported by White (1985) due to abnormal weather conditions causing a large leaching or gaseous loss of N. Under wet conditions very low recoveries of applied N under 40 percent were reported by Van der Meer (1982). Fertilizer N recovery in plants and soil under upland conditions in India rarely exceeds 50 percent (Patra et al., 1992). Increasing the amount of N applied does not increase the amount of N available to the crops, because the fertilizer N is lost before it can be assimilated by the crop (Freney et al., 1992).
Most of the fertilizer N applied to soils is largely in the form of NH4 or NH4‾ producing compounds such as urea and usually oxidized quite rapidly to nitrate. The NO3 formed through nitrification of added N by soil microorganisms is susceptible to loss by leaching and denitrification and may contribute to environmental and surface and ground waters pollution. Thus nitrification acts as a key process in determining fertilizer use efficiency by crops as well as N losses from soils. The adverse effects of oxidation of
The aim of this investigation was to determine the effect of nitrification inhibitor nitrapyrin on N transformations and its impact on recovery of applied N in grassland soil under laboratory conditions.
Materials and Methods
The site, which was almost level and in permanent pasture, situated adjacent to Blaendolau playing fields about 1 km from Soil Science Unit, University of Wales Aberystwyth, UK. The soil was a Dystric Eutrochrept and classified as Conway series (Grid Ref. SN 597804) as described in detail in an earlier study (Abbasi and Adams, 1998).
Fig. 1: | Effect of nitrification inhibitor nitrapyrin on the recovery of N applied to intact soil cores during 42 days incubation. Vatical bars indicate least significant difference (LSD) between with and without nitrapyrin treatments at different time intervals. |
Some physical and chemical characteristics of the soil used are presented in Table 1. Seventy intact soil cores (7.0 cm dia. x 7.5 cm deep) were collected according to the method described in Abbasi and Adams (1998).
Table 1: | Some chemical and physical properties of the soil used during the study Depth (cm) |
Table 2: | Effect of nitrification inhibitor nitrapyrin on the concentration of |
*LSD represent the least significant difference (p≤0.051 appropriate to comparisons between the values within each column and rows |
Table 3: | Effect of nitrification inhibitor nitrapyrin on the concentration of |
*LSD represent the least significant difference (p<0.05) appropriate to comparisons between the values within each column and rows |
The moisture content of soil cores held within brass sampling rings, was adjusted at 70 percent WFPS by saturating and then equilibrating at -5 kPa over 3 days on a tension table. The soil cores were arranged into four treatments i.e. T1 = control, T2 = control with nitrapyrin, T3 =
Triplicate cores from each treatment were removed from the incubator at the beginning and after 7, 14, 28 and 42 days of incubation and sectioned into 0-2.5, 2.5-5.0 and 5.0-7.5 cm layers. Each section was broken down separately and mixed thoroughly. The concentration of total mineral N and
Results and Discussion
Effect of inhibitor on mineral N of the control soil: Initially at the start of the experiment the concentration of
Effect of inhibitor on mineral N of N added soil: In the soil without nitrapyrin,
The concentration of
Effect of inhibitor on recovery of applied N: Data regarding the recovery of applied N (Fig. 1) showed that in the soil cores without nitrapyrin added N was lost continuously after day 7 till the end of incubation. By that time, 53 percent of the N initially present was lost from the system. The rate of N loss was greatest at day 14 and 42, equivalent to more than 1 kg1 day1. By contrast, 17 percent of the applied N was unaccounted for from the soil cores treated with nitrapyrin. Taking these losses into account, over 42 days about 83 percent of the added N was recovered in the nitrapyrin treatment compared with 47 percent recovery in soil cores without nitrapyrin. Nitrapyrin increased the apparent recovery of applied N and of the total amount of N applied 36 percent more N was recovered relative to soil without nitrapyrin. Previous work had shown that ammonia volatilization and leaching were not important pathways for N loss (Abbasi et al., 1997). Consequently it was concluded that major losses were due to denitrification of accumulated
Nitrogen is usually the plant nutrient most limiting in arid and semiarid ecosystems that have a limited input of N and must rely on a controlled and efficient N cycle for continued plant growth and ecosystem function (Bolton et al., 1990). The fact that nitrapyrin conserved
Acknowledgments
The authors would like to thanks the Government of Pakistan for financial support, the University of Jammu & Kashmir Muzafferabad for nominating the senior author for post graduate scholarship.