Abstract: Isolates of Ascochyta rabiei were collected from different chickpea growing areas of Pakistan. The isolates were tested for virulence on two chickpea cultivars ILC-3297 (resistent) and ILC-1929 (susceptible). These isolates were found to synthesize different amounts of phytotoxins Solanapyrone A and C. The virulence of the isolates were found dependent upon the amounts of phytotoxins produced by these isolates.