INTRODUCTION
The Chironomidae, known popularly as non-biting midges are considered
the most widely distributed and abundant family of aquatic insects (Mousavi
et al., 2003). Adult chironomids are most evident when forming
conspicuous swarms, hovering over bushes, trees or other proud markers,
before dusk on summer days. The developmental stages of Chironomidae are
typically aquatic and benthic. Larvae often represent a major component
of the multi-plate fauna in all types of waters and are used frequently
as bio-indicators of ecosystem health. Also, they are playing an important
role in detritus processing and food chains (Garcia and Suárez,
2007).
The country of Egypt, although important for its location, serving as
a corridor between the Palaearctic and the Afro tropical regions, presently
offers little knowledge of its chironomids fauna. The most old publications
(Kieffer, 1925; Freeman, 1958; Steyscal and El-Bialy, 1967) are now outdated.
While the present knowledge of taxonomy, ecology and biology of the Egyptian
chironomid fauna remain limited, there are efforts to remedy the situation
(Zalat et al., 1992; Ghonaim et al., 2001a, b, 2004, 2005a,
b). The purpose of the present study is to add some knowledge on the taxonomy
of this family, which has not fully studied in Egypt.
MATERIALS AND METHODS
The Study Area
El-Tall El-Keber wastewater treatment plant was designed to serve
the population of El-Tall El-Keber city (Fig. 1). Wastewater
is pumped to the plant from a pumping station located outside the plant.
The station was built on five feddans by the Egyptian government; it lies
in the Eastern South of El-Tal El-Kbeer city (Fig. 2).
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Fig. 1: |
Map of El-Tal El-Kaber city |
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Fig. 2: |
El-Tal El-Kaber wastewater treatment plan |
The exact areas that feed the station are El-Tal El-Sagheer, Abd Al Dayem
suburb, El-Tal City and north of rail way station. The output water runs
through Al Wadi drain from El-Tal El-Kbeer to Ismailia waterway to reduce
the under ground water level.
Sample Collection and Preparation
Field investigations were conducted monthly from September 2003 to
August 2004. Chironomid larvae were collected by short sweeps at depth
up to about 1 m from inlet and outlet lagoons. They sorted live, separated
from the sludge and returned to the laboratory in plastic buckets with
some field water.
In the laboratory, each sample was washed by distilled water and sorted
under the microscope, some larvae were fixed in 70% alcohol with few drops
of glycerin for identification by dissection of head capsule, the remaining
sorted larvae were reared to the adult stage as the methods described
by Chattopadhyay (1991).
Identification and Preservation
Identifications were mainly based on available keys (Epler, 2001;
Ghonaim et al., 2001a, b, 2004, 2005a, b) with updates from more
recent available literature. The examination and drawing of specimens
were made by using a binocular microscope and a camera Lucida. Identification
of each species was based primarily on reared adult male specimens; then
females, larvae and pupae were later identified by association with males.
Dissection of the Head Capsule
The mouth parts are of great importance in the identification of chironomid
species. Therefore, larval specimens were dissected for extraction and
examination of head capsule and mouth parts. Then mouth parts were mounted
on cleaned slides using the technique described by Epler (2001).
RESULTS AND DISCUSSION
The field-collected larvae simply cannot be identified with certainty.
The most desirable procedure for associating larvae with their respective
pupae and adult is by rearing. A successfully reared individual yields
all the larval, pupal and adult characters for that particular species.
Confirmation of species identifications by rearing larvae is important
to ensure the integrity of chironomid database (Watts and Pascoe, 1996).
The identification of the collected species depends on the rearing mechanism
and studying the characters of all stages.
Chironomids as biomonitors should prove to be quite valuable once taxonomy
is improved and environmental requirements are defined at the species
level. If this were done, the accurate species lists and identification
keys would be valuable tools in the biological assessment of water quality
(Seire and Pall, 2000). According to this study Two genera: Chironomus
Meigen and Kiefferulus Goetghbuer and six species: C. calipterus
Kieffer, C. formosipennis Kieffer, C. caffrarius Kieffer,
C. imicola Kieffer, C. seydeli Goetghebuer and K. brevibucca
(Kieffer) are now confirmed to the Egyptian fauna. Outline drawings and
pictorial keys are given for the identification of larvae, pupae and adults
of the collected species (Fig. 3-20).
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Fig. 3: |
Pictured key to the studied species (characters of adult
male) |
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Fig. 4: |
Pictured key to Chironomus species (characters
of adult male) |
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Fig. 5: |
Pictured key to the studied species (larval characters) |
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Fig. 6: |
Pictured key to Chironomus species (larval characters) |
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Fig. 7: |
Pictured key to the studied species (pupa characters) |
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Fig. 8: |
Pictured key to Chironomus species (pupal characters) |
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Fig. 9: |
Chironomus calipterus. Adult: (a) head, (b) dorsal
view of thorax, (c) lateral view of thorax, (d) wing, (e) fore tarsomeres,
(f) hypopygium of male and (g) female genitalia |
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Fig. 10: |
Chironomus calipterus. Larva: (a) antennae, (b)
pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla, (f)
mentum, (g) ventromental plate, Pupa: (h) frontal plate, (i) thoracic
horn, (j) shagreen of tergite IV, (k) VIII spur and (l) anal lobe |
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Fig. 11: |
Chironomus formosipennis. Adult: (a) head, (b)
dorsal view of thorax, (c) lateral view of thorax, (d) wing, (e) fore
tarsomeres, (f) hypopygium of male and (g) female genitalia |
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Fig. 12: |
Chironomus formosipennis. Larva: (a) antennae,
(b) pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla,
(f ) mentum, (g) ventromental plate; Pupa: (h) frontal plate, (i)
thoracic horn, (j) abdominal tergites, (k) shagreen of tergites II
and III, (l) VIII spur and (m) anal lobe |
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Fig. 13: |
Chironomus caffrarius. Adult: (a) head, (b) dorsal
view of thorax, (c) lateral view of thorax, (d) wing, (e) fore tarsomeres,
(f) hypopygium of male and (g) female genitalia |
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Fig. 14: |
Chironomus caffrarius. Larva: (a) antennae, (b)
pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla, (f)
mentum, (g) ventromental plate; Pupa: (h) frontal plate, (i) thoracic
horn, (j) shagreen of tergites II, III, IV and V, (k) VIII spur and
(l) anal lobe |
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Fig. 15: |
Chironomus imicola. Adult: (a) head, (b) dorsal
view of thorax, (c) lateral view of thorax, (d) wing, (e) fore tarsomeres,
(f) hypopygium of male, (g) female genitalia and (h) egg sac |
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Fig. 16: |
Chironomus imicola. Larva: (a) antennae, (b)
pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla, (f)
mentum, (g) ventromental plate; Pupa: (h) frontal plate, (i) thoracic
horn, (j) abdominal tergites, (k) shagreen of tergites II, III, IV
and V, (l) VIII spur and (m) anal lobe |
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Fig. 17: |
Chironomus seydeli. Adult: (a) head, (b) dorsal
view of thorax, (c) lateral view of thorax, (d) wing, (e) fore tibia,
(f) fore tarsomeres, (g) hypopygium of male and (h) female genitalia |
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Fig. 18: |
Chironomus seydeli. Larva: (a) antennae, (b)
pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla, (f)
mentum, (g) ventromental plate; Pupa: (h) frontal plate, (i) thoracic
horn, (j) shagreen of tergites III and IV, (k) VIII spur and (l) anal
lobe |
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Fig. 19: |
Kifferulus brevibucca. Adult: (a) head, (b) dorsal
view of thorax, (c) lateral view of thorax, (d) wing, (e) fore tarsomeres,
(f) hypopygium of male and (g) female genitalia |
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Fig. 20: |
Kifferulus brevibucca. Larva: (a) antennae, (b)
pecten epipharyngis, (c) premandible, (d) mandible, (e) maxilla, (f)
mentum, (g) ventromental plate; Pupa: (h) frontal plate, (i) thoracic
horn, (j) shagreen of tergites II and III, (k) VIII spur and (l) anal
lobe |