Induction of Gene Expression Alterations by Culture Medium from Trypsinized Cells
Abstract:
This study hypothesized that trypsin treatment itself could be a stress inducer before any other physical or chemical mediated stress is introduced. To further understand the role of trypsin treatment, we incubated adherent cells with conditioned growth medium isolated from trypsinized cells after several hours of trypsin action and examined global gene expression profile with microarray technology. Microarray data identified large-scale gene expression alterations in cells receiving conditioned medium from trypsin treated cells compared to control cells that did not receive such medium. Twenty eight genes were found to be upregulated with at least two-fold change in the expression level, while 70 genes were downregulated. Gene expression signature clearly identified stress response. Taken together this data cautions the contribution of background stress while assessing the effects of radiation, certain drugs or environmental mutagens. Further attention is required while determining the role of conditioned medium in elucidating radiobiological phenomenon such as bystander effect.
How to cite this article
M. Ahmad Chaudhry , 2008. Induction of Gene Expression Alterations by Culture Medium from Trypsinized Cells. Journal of Biological Sciences, 8: 81-87.
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