Abstract: The present study was conducted to evaluate the analgesic, antioxidant and anti-inflammatory effects of two chalcones, 21-hydroxy-2,41-dimethoxychalcone [2-DMC] and 4-hydroxychalcone [4HC] synthesized in our laboratory. Antioxidant property of the two chalcones were compared with ascorbic acid and were evaluated in the 1,1-diphenyl-2-picryl-hydrazyl (DPPH) free radical assay. Results showed a potent analgesic effect of the two chalcones at 50, 100 and 200 mg kg-1 intraperitoneally. A significant inhibitions in acetic acid-induced abdominal contractions and significant percentage increase in pain threshold in hot-plate test were exhibited. However in flick test, highest analgesic activity was observed only with 4HC (200 mg kg-1, i.p.) which exhibited 91.9% increase in pain threshold even more than the standard drug, acetylsalicylic acid that produced 85.0%. The % antioxidant activity (AA) of 2-DMC and 4HC ranges between 14.42- 48.99 and 17.09 -24.83%. AA of 2-DMC and 4HC were shown to be long and short acting respectively with the time period of 20 min. The anti-inflammatory effects of the two chalcones of both 2-DMC and 4HC produced% inhibitions between 27.0, 49.2, 78.9% and 30.2, 49.2, 77.8% respectively, while indomethacin gave 28.6% in oedema formation of mice right hind paw. In pulmonary oedema and leucocytes count from the mice pleurisy, only 200 mg kg-1 i.p of the chalcones produced significant anti-inflammatory effect. This study demonstrated that the inhibitory effects of 2-DMC and 4HC on various mediators responsible for pain and inflammation and indicated the effectiveness of the chalcones as potent analgesic, antioxidant and anti-inflammatory agents.