Antimicrobial and Antiulcer Activities of Methanol Extract of Allium sativum on Helicobacter pylori
Abstract:
Methanol extract of Allium sativum was investigated for in vitro antibacterial activity against H. pylori using standard strain ATCC 43504 and three clinical isolates (UCH97009, UCH99041 and UCH99052) and also for anti ulcer effect in rat. All the strains except UCH99041 were inhibited by thefinal concentration of the tested extract (6 mg mL-1) to varying degrees. The Minimum Inhibitory Concentration (MIC) against all susceptible strains ranged between 153 and 625 μg mL-1. Urease activity of both UCH97009 and UCH99052 strains decreased with increase in the concentration of the extract while the cell surface hydrophobicity of two of the strains was reduced by the extract as there was no aggregation of the cell by the extract. SAT titre was >3.0 M. Methanol extract of A. sativum reduced mean ulcer score from 13.63±1.33 in control to 10.06±1.27 in extract treated. The anti- ulcer effect was however not significant (p>0.05). The acute toxicity test (intraperitonial administration) methanol extract in mice gave LD50 of 8.7 g kg-1 body weight. The result indicates that Allium sativum taken at a low dose may have some therapeutics potentials against gastric ulcers associated with H. pylori infection.
How to cite this article
B. A. Adeniyi, F.S. Oluwole and F. M. Anyiam, 2006. Antimicrobial and Antiulcer Activities of Methanol Extract of Allium sativum on Helicobacter pylori. Journal of Biological Sciences, 6: 521-526.
REFERENCES
Adeniyi, B.A., 1996. A study of antimicrobial properties of extracts from Diospyros mespiliformis HOESTH (EBENACEAE). Ph.D. Thesis, University of Ibadan.
Adeniyi, B.A. and F.M. Anyiam, 2004. In vitro anti-Helicobacter pylori potential of methanol extract of Allium ascalonicum leaf: Susceptibility and effect on urease activity. Phytother. Res., 18: 358-361.
Direct Link
Alphin, R.S. and J.W. Ward, 1967. Actions of hexopyrronium bromide on gastric secretion in dogs and on gastric secretion and ulceration in rats. Arch. Int. Pharmacodyn. Ther., 168: 82-100.
PubMed
Annuk, H., S. Hirmo, E. Turi, M. Mikelsaar, E. Ara and T. Wadstrom, 1999. Effect on cell surface hydrophobicity and susceptibility of Helicobacter pylori to medicinal plant extracts. FEMS Microbiol. Lett., 172: 41-45.
PubMed Direct Link
Chuag, J.G., G.W. Chem, L.T. Chang, J.G. Lin, C.C. Yeh and J.F. Wang, 1998. Effects of garlic compounds- diallyl sulphide and diallyl disulphite on arylamine N-acetyltransferase activity in strains of Helicobacter pylori from peptic ulcer patients. Am. J. Chin. Med., 26: 353-364.
Diker, K.S. and G. Hascelik, 1994. The Bactericidal activity of tea against Helicobacter pylori. Lett. Applied Microbiol., 19: 299-300.
CrossRef Direct Link
Gadhi, C.A., A. Benharref, M. Jana and A. Lozniewski, 2001. Anti Helicobacter pylori activity of Aristolochia paucinervis pomel extracts. J. Ethnopharmacol., 75: 203-205.
CrossRef
Gold, B.D., 1999. Current therapy for Helicobacter pylori infection in children and adolescents. Can. J. Gastroenterol., 13: 571-579.
Direct Link
Graham, D.Y., S.Y. Anderson and T. Lang, 1999. Garlic of jalapeno peppers for treatment of Helicobacter pylori infections. Am. J. Gastroterol., 94: 1200-1202.
Direct Link
Hamilton-Miller, J.M.T. and R.A. Gargan, 1979. Rapid screening of urease inhibitors. Investigative Urol., 16: 327-328.
Direct Link
Keigo, S., D. Yohei, S. Naohiro, Y. Tetsuya, N. Toshi, I. Yoshitsugu and A. Yosichika, 2001. Apoptotic signaling pathways >activated by Helicobacter pylori infection and increase of Apoptosis-inducing Activity under serum-starved conditions. Infect. Immun., 9: 3181-3189.
CrossRef Direct Link
Lajubutu, B.A., R.J. Pinney, M.F. Roberts, H.A. Odelola and B.A. Oso, 1995. Antibacterial activity of diosquinone and plumbagin from the root of Diospyros mespiliformis (Hostch) (Ebenaceae). Phytother. Res., 9: 346-350.
CrossRef Direct Link
Ljungh, A.I., A. Brown and T. Wadstroem, 1985. Surface Hydrophobicity of Coagulase Negative Staphylococci Determined by Salt Aggregation Test. In: The Staphylococci, Jeljaszewicz, J. (Ed.). Gustav Fischer Verlag Stuttgart, New York, pp: 157-161
Mahady, G.B., S.L. Pendland, G.S. Yun, Z.Z. Lu and A. Stonia, 2003. Ginger (Zingiber officinale Roscoe) and the gingerols inhibit the growth of Cag A+ strains of Helicobacter pylori. Anticancer Res., 23: 3699-3702.
PubMed
Marshall, B.J. and J.R. Warren, 1984. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet, 11: 1311-1315.
CrossRef Direct Link
Mobley, H.L.T., M.J. Coresia, L.E. Rosenthal and B.D. Jones, 1988. Characterization of urease from Campylobacter pylori. J. Clin. Microbiol., 26: 831-836.
Direct Link
Nariman, F., F. Eftekhar, Z. Habibi and T. Falsafi, 2004. Anti-Helicobacter pylori activities of six Iranian plants. Helicobacter, 9: 146-151.
Direct Link
O'Gara, E.A., D.J. Hill and D.J. Maslin, 2000. Activities of garlic oil, garlic powder and their diallyl constituents against Helicobacter pylori. Applied Environ. Microbiol., 66: 2269-2273.
CrossRef Direct Link
Sivam, G.P., J.W. Lampe, B.S. Ulness, S.R. Wanzy and J.O. Potter, 1997. Helicobacter pylori in vitro susceptibility to garlic (Allium sativum) extracts. Nutr. Cancer, 27: 118-121.
Direct Link
Tabak, M., R. Armon, I. Potasman and I. Neeman, 1996. In vitro inhibition of Helicobacter pylori by extracts of thyme. J. Applied Bacteriol., 80: 667-672.
Direct Link
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