HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2006 | Volume: 6 | Issue: 1 | Page No.: 193-197
DOI: 10.3923/jbs.2006.193.197
Process Optimization for ß-D-Galactosidase Production Using Yeast Culture
R. Panesar, P.S. Panesar, R.S. Singh, J.F. Kennedy and M. B. Bera

Abstract: The use of Kluyveromyces marxianus NCIM 3465 for the production of ß-D-galactosidase is reported. Experimentation was carried out to optimize the process parameters for maximum enzyme production by varying the parameters such as pH of the medium, temperature, inoculum size, age of inoculum, agitation and incubation time. The maximum enzyme activity was observed with pH 5.0, temperature 30°C, inoculum size 6% (v/v) having 20 h age, under shaking conditions (100 rpm) after 24 h of incubation.

Fulltext PDF

How to cite this article
R. Panesar, P.S. Panesar, R.S. Singh, J.F. Kennedy and M. B. Bera, 2006. Process Optimization for ß-D-Galactosidase Production Using Yeast Culture. Journal of Biological Sciences, 6: 193-197.

Keywords: Yeast, �-D-galactosidase and enzyme activity

REFERENCES
  • Mahoney, R.R., 1997. Lactose Enzymatic Modification. In: Advanced Dairy Chemistry, Fox, P.F. (Ed.). Chapmann and Hall, London, UK., pp: 77-125

  • Coughlin, R.W. and M. Charles, 1980. Applications of Lactase and Immobilized Lactase. In: Immobilized Enzymes for Food Processing, Pitcher, W.H. (Ed.). CRC Press, Florida, USA., pp: 153-179

  • Manjon, A., F.I. Llorca, M.J. Bonete, J. Bastida and J.L. Iborra, 1985. Properties of β galactosidase covalently immobilized to glycophase coated porous glass. Process Biochem., 20: 17-22.
    Direct Link    

  • Joshi, M.S., L.R. Gowda, L.C. Katwa and S.G. Bhat, 1989. Permeabilization of yeast cells (Kluyveromyces fragilis) to lactose by digitonin. Enzyme Microb. Technol., 11: 439-443.
    CrossRef    

  • Stredansky, M., M. Tomaska, E. Sturdik and L. Kremnicky, 1993. Optimization of galactosidase extraction from Kluyveromyces marxianus. Enzyme Microb. Technol., 15: 1063-1065.

  • Mahoney, R.R., 1998. Galactosyl-oligosaccharide formation during lactose hydrolysis a review. Food Chem., 63: 147-154.
    CrossRef    

  • Miller, J.H., 1972. Experiments in Molecular Genetics. Cold Spring Harbor Laboratory, New York, pp: 352

  • Klovrychev, M.F., P.N. Korolev and V.G. Bulgakova, 1979. Effect of copper ions and unfavourable pH on protein and RNA synthesis of Candida utilis. Microbiology, 47: 357-361.

  • Inchaurrondo, V.A., O.M. Yantorno and C.E. Voget, 1994. Yeast growth and galactosidase production during aerobic cultures in lactose limited synthetic medium. Process Biochem., 29: 47-54.

  • Artolozaga, M.J., R. Jones, A.L. Schneider, S.A. Furlan and M.F.C. Jones, 1998. One step partial purification of β D galactosidase from Kluyveromyces marxianus CDB002 using streamline DEAE. Bioseparation, 7: 137-143.
    CrossRef    

  • Matheus, A.O.R. and N. Rivas, 2003. Production and partial characterization of β-galactosidase from Kluyveromyces lactis grown in deproteinized whey. Arch. Latinoam. Nutr., 53: 194-201.
    PubMed    

  • Hewitt, G.M. and J.W.D. GrootWassink, 1984. Simultaneous production of inulase and lactase in batch and continuous cultures of Kluyveromyces fragilis. Enzyme Microb. Technol., 6: 263-270.

  • Ku, M.A. and Y.D. Hang, 1992. Production of yeast lactase from sauerkraut brine. Biotechnol. Lett., 14: 925-928.
    CrossRef    

  • Champluvier, B., B. Kamp and P.G. Rouxhet, 1988. Preparation and properties of β galactosidase confined in cells of Kluyveromyces sp. Enz. Microb. Technol., 10: 611-617.
    Direct Link    

  • Pedrique, M. and F.J. Castillo, 1982. Regulation of β D galactosidase synthesis in Candida pseudotropicalis. Applied Environ. Microbiol., 43: 303-310.
    Direct Link    

  • Dickson, R.C. and J.S. Markin, 1980. Physiological studies of β galactosidase induction in K. lactis. J. Bacteriol., 142: 777-785.
    Direct Link    

  • Ranzi, B.R., D. Porro, C. Compagno and E. Martegani, 1987. Protein and cell volume distributions during the production of β galactosidase in batch cultures of Kluyveromyces lactis. J. Biotechnol., 5: 227-231.

    • © Science Alert. All Rights Reserved