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Journal of Biological Sciences

Year: 2006 | Volume: 6 | Issue: 6 | Page No.: 1088-1092
DOI: 10.3923/jbs.2006.1088.1092
Characterization of Drug Resistant Staphylococcus aureus of Animal Origin
O. K. Achi and G.A. Ugbogu

Abstract: One hundred and eighty samples for culture of ear and nose swabs collected from livestock at a local animal farm at Umudike were examined to determine the incidence of Staphylococcus aureus. The susceptibility of the isolates to 15 antimicrobial agents allowed for human and animal therapy were also evaluated. The results show that these animals frequently harbour S. aureus strains. In addition, a high number (79%) of the isolates were resistant to one or more antimicrobial agents and 10 different resistance profiles were recorded. Resistance to amoxycillin (71%) and Cotrimoxazole (62.2%) was demonstrated for the isolates. The result indicate that the occurrence of multi-resistant S. aureus strains in farm animals may constitute a reservoir for disseminating antibiotic-resistance in the community and the need for the prudent drugs use to diminish the development and spread of antimicrobial resistance.

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How to cite this article
O. K. Achi and G.A. Ugbogu , 2006. Characterization of Drug Resistant Staphylococcus aureus of Animal Origin. Journal of Biological Sciences, 6: 1088-1092.

Keywords: Staphyloccus aureus, antibiotic resistance, farm animals and incidence

REFERENCES
  • Baird-Parker, A.C., 1979. Methods of Identifying Staphylococci and Micrococci. In: Identification Methods for Microbiologists, Skinner F.A and D.W. Lovelock (Eds). Academic Press, London

  • Bates, J., J.Z. Jordens and D.T. Griffiths, 1994. Farm animals as putative reservoir for vancomycin-resistant entercoccal infections in man. J. Antimicrob. Agents Chemother., 34: 507-516.

  • Caprioli, A., L. Busani, J.L. Martel and R. Helmuth, 2000. Monitoring of antibiotic resistance in bacteria of animal origin: Epidemiological and microbiological methodologies. Int. J. Antimicrob. Agents, 14: 295-301.
    Direct Link    

  • Clark, D.S., 1965. Method of estimating the bacterial population on surfaces. Can. J. Microbiol., 11: 407-413.
    CrossRef    Direct Link    

  • Cole, E.C., R.M. Addison, J.R. Rubino, K.E. Leese and P.D. Dulaney et al., 2003. Investigation of antibiotic and antibacterial agent cross-resistance in target users and nonusers. J. Applied Microbiol., 95: 664-676.

  • Collins-Thompson, D.L., D.S Wood and I.Q. Thompson, 1988. Detection of antibiotic residence in consumer milk supplies in North America using the charm test II procedure. J. Food Prot., 51: 632-633.

  • Devriese, L.A., K.H. Schleifer and G.O. Adegoke, 1985. Identification of coagulase-negative staphylococci from farm animals. J. Applied Bacteriol., 58: 45-55.
    CrossRef    Direct Link    

  • Du Pont, H.L. and J.H. Steels, 1987. Use of antimicrobial agents in animal feeds: Implications for human health. Rev. Infect. Dis., 9: 447-460.
    PubMed    Direct Link    

  • Endtz, H.P., G.I. Ruijs, B. van Klingeren, W.H. Jansen, T. van der Reyden and R.P. Mouton, 1991. Quinolone resistance in Campylobacter isolated from man and poultry following the introduction of fluoroquinolones in veterinary medicine. J. Antimicrob. Chemother., 27: 199-208.
    CrossRef    Direct Link    

  • Forbes, B.A. and D.R. Schaberg, 1983. Transfer of resistance plasmids from Staphyloccus epidermidis to Staphylococcus aureus evidence for conjugative exchange of resistance. J. Bacteriol., 167: 627-634.

  • Franco, D.A., A.C.R. Ghilardi, A.T. Tavechio, A.M.O. Machado and A.C.C. Pignatari, 1990. Antibiotic and sulphonamide residues in meat. Implication for human health. Food Prot., 53: 178-185.

  • Holmerg, S.D., M.T. Osterholm, K.A. Senger and M.C. Cohen, 1984. Drug-resistant Salmonella from animals fed antimicrobials. N. Eng. J. Med., 311: 617-622.

  • Kessie, G., M. Eyahebi, A.M. Haddad and A.M. Shibl, F.J. al-Shammary, A.F. Tawfik and M.N. al-Ahdal, 1998. Plasmid profiles and antibiotic resistance in coagulase negative staphylococci isolated from polluted water. J. Applied Microbiol., 84: 417-422.
    CrossRef    

  • Lowy, F.D., 1998. Staphylococci aureus infections. N. Engl. J. Med., 339: 520-532.

  • Maranan, M.C., B. Moreira, S. Boyels-Vavra and R.S. Daum, 1997. Antimicrobial resistance in staphylococci. Epidemiology, molecular mechanisms and clinical relevance. Infect. Dis. Clin. North Am., 11: 813-849.
    PubMed    

  • Oyekunle, M.A. and A. Adetosoye, 1998. Biochemical characterization of Staphylococcus isolated from Ndama cattle. Trop. Vet., 6: 48-54.

  • Shakibaie, M.R., S. Mansouri and S. Hakak, 1999. Plasmid pattern of antibiotic resistance in lactamase producing S. aureus isolated from hospital in Kerman, Iran. Arch. Iran Med., 2: 1-6.

  • Shoemaker, N.B., H. Vlamkis, K. Hayes and A.A. Sayers, 2001. Evidence for extensive resistance gene transfer among Bacteroides sp. and among other genera in the colon. Applied Environ. Microbiol., 67: 561-568.
    CrossRef    Direct Link    

  • Singer, R.S., R. Finch, H.C. Wegener, R. Bywater, J. Walters and M. Lipsitch, 2003. Antibiotic resistance-the interplay between antibiotic use in animals and human beings. Lancet Infect. Dis., 3: 47-51.
    CrossRef    

  • Smith, D.L., A.D. Harris, J.A. Johnsons, E.K. Silbergeld and J.G. Morris Jr., 2002. Animal antibiotic use has an early but importance in human commensal bacteria. Proc. Natl. Acad. Sci. USA., 99: 6434-6439.

  • Synder, O.P. and D.M. Poland, 1991. America`s Safe Food part 2. Dairy Food, Environ. Sanit., 11: 4-20.

  • Tenover, F.C. and J.M. Hughes, 1996. Development and spread of multiple-resistant bacterial pathogens. J. Am. Med. Assoc., 275: 300-330.

  • WHO., 2001. WHO Global Strategy for Containment of Antimicrobial Resistance. World Health Organization, Geneva

  • Speck, M.E., 1984. Compendium of Methods for the Microbiological Examination of Foods. 2nd Edn., American Public Health Association, Washington DC

  • Bergdoll, M.D., 1989. Staphylococcus aureus. In: Foodborne Bacterial Pathogens, Doyle, M.P. (Ed.). Marcel Dekker Inc., New York, pp: 463-524

  • Collins, C.H., P.M Lyne and J.M. Grange, 1990. Microbiological Methods. 6th Edn., Butterworths, London, pp: 409

  • Levy, S.B., 1984. Antibiotic-Resistant Bacteria in Food and Animals. In: Antimicrobials and Agriculture, Woodbine, M. (Ed.). 4th Edn., University of Nottingham, Butterworths, London

  • NCCLS, 2001. Performance Standards for Antimicrobial Susceptibility Testing 11th Informational Supplement. National Committee for Clinical Laboratory Standards, Wayne, PA, USA

  • Harrigan, W.F. and M.F. McCance, 1976. Laboratory Methods in Food and Dairy Microbiology. 7th Edn., Churchill Livingstone, New York

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