Abstract: In this study, different doses of sulfur (0, 125, 250 and 375 g m 2) and humic acid (0, 10, 20 and 30 g m 2) were incorporated into soil to determine the effects of sulfur (S) and humic acid (HA) on yield components and nutrient contents in spinach. The applications of HA increased total yield of spinach. The relationship between increasing doses of S and HA with the total yield was found significant (p<0.05). But the relationships between doses of S and HA with yield components of spinach were not significant statistically. According to the results, increasing doses of S and HA increased N and P contents in plants. The relationships between HA doses and N content of plant were found significant (p>0.05). But, none of the application did affect K, Ca and Mg contents in plants statistically.