Aseptic Multiplication and Maintenance of Bitter Gourd (Momordica
charantea Linn.) as Affected by Sucrose, Agar and pH
Abstract:
Effects of different concentrations of sucrose, agar and different levels of
pH on in vitro axillary shoot multiplication of Momordica charantea
Linn. were studied in the present investigation. The nodal segments from the
field grown plant were used as testing plant material. For maximum number of
shoot induction and multiplication in MS medium �containing 2.0 mg L 1
BAP+0.2 mg L 1 NAA with 30 g L 1 of sucrose, 7 g L 1 of
agar and 5.5-6.0 levels of pH proved more effective. When the medium having 30 g
L 1 sucrose, the highest percentage of explant responded to shoot
proliferation and that was 100%. This sucrose concentration also showed the
optimum result for total number of shoot per culture and average length of
shoots and that were 5.1�0.8 and 5.6�0.4 cm, respectively. The highest
response of shoot proliferation from the nodal explant was observed on MS medium
having 7 g L 1 of agar and the frequency was 100%. Among different
levels of pH, the highest percentage of explant showing proliferation was
observed on the media adjusted to pH 5.5-6.0 levels. It was proved by the
present investigation that in vitro growth and shoot multiplication was
affected by sucrose, agar and pH on the shoot induction medium.
How to cite this article
R. S. Sultana, M. A. Bari Miah, M. M. Rahman, M. H. Rahman and M. U. Mollah, 2005. Aseptic Multiplication and Maintenance of Bitter Gourd (Momordica
charantea Linn.) as Affected by Sucrose, Agar and pH. Journal of Biological Sciences, 5: 781-785.
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