Sweet Cherry Cultivar Identification by Using SSR Markers
Abstract:
In this study, sweet cherry varieties and types grown in Turkey were
described using SSR markers. This study was undertaken to develop DNA marker
profiles that could be used to distinguish among the sweet cherry cultivars used
in production in Turkey. For microsatellite analysis, 13
SSR primers isolated from sweet cherry (P. avium L.) sour cherry (P.
cerasus L.) and peach (P. persica L. Batsch) were used on sweet
cherry cultivars and types. Two primer pairs did not give amplification with
genotypes analyzed. Two-primer pairs amplified monomorphic fragment for the
sweet cherry varieties therefore they were uninformative for the sweet cherry
genetic analysis. Genetic similarities were calculated and a dendrogram has been
established. All of the 8 SSR primers used and cherries have produced amplified
bands. For each primer the alleles obtained has been between 1 and 6, in total
38 alleles have found. Through these analyses the similarities between these
varieties have been converted into numerical values. This happens to be the
first study kind towards the molecular identification of sweet cherry genetic
resources in Turkey.
How to cite this article
Y ld z Aka Kacar, Amy Iezzoni and Selim Cetiner, 2005. Sweet Cherry Cultivar Identification by Using SSR Markers. Journal of Biological Sciences, 5: 616-619.
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