HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2005 | Volume: 5 | Issue: 4 | Page No.: 424-426
DOI: 10.3923/jbs.2005.424.426
Genetic Similarity by RAPD Between Pure Lines of Chickens
A. Okumus and M. Kaya

Abstract: Twelve primers were used to characterize the genetic similarity among ten chickens which named RIR I, RIR II, Barred I, Barred II, Colombian Rock, Line-54, Blue Line, Maroon Line, Black Line and Brown Line provided by Ankara Poultry Research Institute-Turkey. The objective of this study were to determine PCR-based genetic similarity between lines. Totally, 35 bands were scored for the dendogram prepared on the bases of a similarity matrix using the UPGMA algorithma. The highest and lowest genetic similarities were found to be 0.3773 between Bar I and Maroon and 0.0899 between Col and Bar II, respectively.

Fulltext PDF

How to cite this article
A. Okumus and M. Kaya, 2005. Genetic Similarity by RAPD Between Pure Lines of Chickens. Journal of Biological Sciences, 5: 424-426.

Keywords: pure line, genetic similarity, RAPD, Chicken and dendogram

REFERENCES
  • Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey, 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res., 18: 6531-6535.
    CrossRef    PubMed    Direct Link    

  • Bujo, H., R.G. Elkin, K.A. Lindstedt, J. Nimpf, J.J. Bitgood and W.J. Scheider, 1996. A rapid PCR-based procedure for idendifying mutant restricted ovulator chickens. Poult. Sci., 75: 1113-1117.

  • Hedrick, P., 1992. Shooting in RAPDs. Nature, 335: 679-680.

  • Smith, E.J., C.P. Jones, J. Bartlett and K.E. Nestor, 1996. Use of Randomly Amplified Polymorphic DNA markers for the genetic analysis of relatedness and diversity in chickens and turkeys. Poult. Sci., 75: 579-584.
    PubMed    Direct Link    

  • Sharma, D., R.K.B. Appa, R.V. Singh and S.M. Totey, 2001. Genetic diversity among chicken breeds estimated through randomly amplified polymorphic DNA. Anim. Biotechnol., 12: 111-120.
    CrossRef    PubMed    Direct Link    

  • Sharma, D., K.B.C.A. Rao and S.M. Totey, 2000. Measurement of within and between population genetic variability in quails. Br. Poult. Sci., 41: 29-32.
    CrossRef    PubMed    Direct Link    

  • Levin, I., L.B. Crittenden and J.B. Dodgson, 1993. Genetic map of the Z chromosome using Random amplified polymorphic DNA markers. Genomics, 16: 224-230.

  • Wei, R., M.R. Dentine and J.J. Bitgood, 1997. RAPD Markers in crosses between inbred lines of Rhode Island Red and White Leghorn chicken. Anim. Genet., 28: 291-294.
    Direct Link    

  • Nei, M. and W.H. Li, 1979. Mathematical modelling for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA., 76: 5269-5273.

  • Singh, R.V. and D. Sharma, 2002. Within- and between-strain genetic variability in White Leghorn population detected through RAPD markers. Br. Poult. Sci., 43: 33-37.
    CrossRef    PubMed    Direct Link    

  • Plotsky, Y., M.G. Kaiser and S.J. Lamont, 1995. Genetic characterization of highly inbred chicken lines by two DNA methods: DNA fingerprinting and PCR reaction using arbitrary primers. Anim. Genet., 26: 163-170.

  • Ali, B.A., M.M. Ahmed and O.M. Aly, 2003. Relationship between genetic similarity and some productive traits in local chicken strains. Afr. J. Biotechnol., 2: 46-47.
    Direct Link    

  • Sharma, D., K.B.C.A. Rao and S.M. Totey, 1998. Estimation of genetic diversity among various breeds of poultry using randomly amplified polymorphic DNA. Proceedings of the10th European Poultry Conference, (PEPC,98), Israel, pp: 259-262.

  • Zhang, X., F.C. Leung, D.K.O. Chan, G. Yang and C. Wu, 2000. Genetic diversity of Chinese native chicken based on allozyme, randomly amplified polymorphic DNA and microsatellite polymorphism. Proceedings of the 21th Worlds Poultry Congress, Aug. 20-24, Canada, pp: 1463-1472.

  • Ali, B.A. and M.M.M. Ahmed, 2001. Random amplified polymorphic DNA in some chicken strains. Proceedings of the 1st Conference of Role of Biochemistry in Environment and Agriculture, February 6-8, 2001, Cairo, Egypt, pp: 23-31.

    • © Science Alert. All Rights Reserved