HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2005 | Volume: 5 | Issue: 3 | Page No.: 330-334
DOI: 10.3923/jbs.2005.330.334
Some Properties of Inulinase from Rhizoctonia solani
Figen Ertan, Filiz E. Sanal, Aysegul C. Kaboglu, Tulin Aktac and Elvan Bakar

Abstract: The preparation and some biochemical properties of inulinase from R. solani which isolated from soil in Tekirdag - Turkey, was carried out by dialysis and concentrated with silica gel-60 then, further proceeded with Sephadex G-150 and DEAE-Cellulose chromatographies. The specific activity of the enzyme was enhanced from 0.256-5.43 U mg -1. The enzyme showed maximum activity at 35°C and pH 5.0, it was stable in the pH range of 5.0-6.5 and up to 40°C. The activity of enzyme was inhibited strongly by Hg2+ and Ag2+ and KCN and DTNB. The action mode of enzyme for inulin was determined as endohydrolitic-action by TLC.

Fulltext PDF

How to cite this article
Figen Ertan, Filiz E. Sanal, Aysegul C. Kaboglu, Tulin Aktac and Elvan Bakar, 2005. Some Properties of Inulinase from Rhizoctonia solani. Journal of Biological Sciences, 5: 330-334.

Keywords: fructooligosaccharide, properties, endo-action, R. solani and Inulnase

REFERENCES
  • Park, J.P. and J.W. Yun, 2001. Utilisation of chicory roots for microbial endoinulinase production. Lett. Applied Microbiol., 33: 183-187.

  • Ohta, K., S. Hamada and T. Nakamura, 1993. Production of high concentrations of ethanol from inulin by simultaneous saccharification and fermentation using Aspergillus niger and Saccharomyces cerevisiae. Applied Environ. Microbiol., 59: 729-733.

  • Arand, M., A.M. Golubev, J.R.B. Neto, I. Polikarpov and R. Wattiez et al., 2002. Purification, characterisation, gene cloning and preliminary X-ray data of exo-inulinase from Aspergillus awamori. Biochem. J., 362: 131-135.

  • Nakamura, T., S. Hamada, Y. Nishino and K. Ohta, 1994. Occurrence of two forms of extracellular endoinulinase from Aspergillus niger mutant 817. J. Ferment. Bioeng., 78: 134-139.

  • Vandamme, E.J. and D.G. Derycke, 1983. Microbial inulinases: Fermentation process, properties and application. Adv. Applied Microbiol., 29: 139-176.
    PubMed    

  • Viswanathan, P. and P.R. Kulkarni, 1995. Effect of polyols on heat inactivation of Aspergillus niger van teghem inulinase. Lett. Applied Microbiol., 21: 282-284.

  • Gupta, A.K., A. Gill and N. Kaur, 1998. A HgCl2 insensitive and thermally stable inulinase from Aspergillus oryzae. Phytochemistry, 49: 55-58.
    CrossRef    

  • Kochhar, A., N. Kaur and A.K. Gupta, 1997. Inulinase form Aspergillus versicolor: A potent enzyme for producing fructose from inulin. J. Sci. Ind. Res., 56: 721-726.
    Direct Link    

  • Ongen-Baysal, G., S. Sukan and N. Vassilev, 1994. Production and properties of inulinase from Aspergillus niger. Biotechnol. Lett., 16: 275-280.

  • Nguyen, Q.D., F. Mattes, A. Hoschke, J. Rezessy-Szabo and M.K. Bhat, 1999. Production, purification and identification of fructooligosaccharides produced by β-fructofuranosidase from Aspergillus niger IMI 303386. Biotechnol. Lett., 21: 183-186.

  • Xiao, R., M. Tanida and S. Takao, 1988. Inulinase from Chrysporium pannarum. J. Ferment. Technol., 66: 553-558.

  • Miller, G.L., 1959. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31: 426-428.
    CrossRef    Direct Link    

  • Lowry, O.H., N.J. Rosebrough, A.L. Farr and R.J. Randall, 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem., 193: 265-275.
    CrossRef    PubMed    Direct Link    

  • Azhari, R., A.M. Szlak, E. Ilan, S. Sideman and N. Lotan, 1989. Purification and characterization of endo-and exo-inulinase. Biotechnol. Applied Biochem., 11: 105-117.

  • Barthomeuf, C., F. Regerat and H. Pourrat, 1991. Production of inulinase by a new mold of Penicillium rugulosum. J. Ferment. Bioeng., 72: 491-494.
    CrossRef    Direct Link    

  • Kato, K., T. Araki, T. Kitamura, N. Morita, M. Moori and Y. Suzuki, 1999. Purification and properties of a thermostable inulinase (β-D-Fructan Fructohydrolase) from Bacillus stearothermophilus KP1289. Starch, 51: 253-258.
    Direct Link    

  • Kushi, R.T., R. Monti and J. Contiero, 2000. Production, purification and characterisation of an extracellular inulinase from Kluyveromyces marxianus var. bulgaricus. J. Ind. Microbiol. Biotechnol., 25: 63-69.

  • Onodera, S. and N. Shiomi, 1988. Purification and substrate specificity of endo-type inulinase from Penicillium purpurogenum. Agric. Biol. Chem., 52: 2569-2576.

  • Xiao, R., M. Tanida and S. Takao, 1989. Purification and characteristics of two exoinulinases from Chrysosporium pannorum. J. Ferment. Bioeng., 67: 331-334.

  • Haraguchi, K., K. Hayashi and T. Kasumi, 1990. Purification and some properties of endoinulinase from Chysosporium pannorum. J. Ferment. Bioeng., 67: 244-248.

  • Uhm, T.B. and S.M. Byun, 1987. Thermal stability of the multiple charge isoform of inulinase from Aspergillus niger. Biotechnol. Lett., 9: 287-290.

  • Efstathiou, I., G. Reysset and N. Truffaut, 1986. A study of inulinase activity in the Clostridium acetobutylicum Strain AKBn8. Applied Microbiol. Biotechnol., 25: 143-149.

  • Gupta, A.K., D.P. Singh, N. Kaur and R. Singh, 1994. Production, purification and immobilization of inulinase from Kluyveromyces fragilis. J. Chem. Technol. Biotechnol., 59: 377-385.
    CrossRef    Direct Link    

  • Parekh, S. and A. Margaritis, 1985. Inulinase (β-fructofuranosidase) production by Kluyveromyces marxianus in bath culture. Applied Microbiol. Biotechnol., 22: 446-448.

  • Pessoni, R.A.B., R.L.C. Figueiredo-Ribeiro and M.R. Braga, 1999. Extracellular inulinases from Penicillium janczewskii, a fungus isolated from the rhizosphere of Vernonia herbacea (Asteraceae). J. Applied Microbiol., 87: 141-147.
    CrossRef    

  • Ettalibi, M. and J.C. Baratti, 1990. Molecular and kinetic properties of Aspergillus ficuum inulinases. Agric. Biol. Chem., 54: 61-68.

  • Nakamura, T., S. Maruki, S. Nakatsu and S. Ueda, 1978. General properties of extracellular inulinase (PII) from Aspergillus sp. (studies on microbial inulase II). Nippon Nogeikagaku Kaishi, 52: 581-587.

  • Onodera, S. and N. Shiomi, 1992. Purification and subsite affinities of exoinulinase from Penicillium trzebinskii. Biosci. Biotechnol. Biochem., 56: 1443-1447.

  • Nakamura, T., A. Shitara, S. Matsuda, T. Matsuo, M. Suiko and K. Ohta, 1997. Production, purification and properties of an endoinulinase of Penicillium sp. TN-88 that liberates inulotriose. J. Ferment. Bioeng., 84: 313-318.
    CrossRef    

  • Yun, J.W., D.H. Kim, B.W. Kim and S.K. Song, 1997. Production of inulo-oligosaccharides from inulin by immobilised endoinulinase from Pseudomonas sp. J. Ferment. Bioeng., 84: 369-371.
    Direct Link    

  • Uhm, T.B., M.S. Chung, S.H. Lee, F. Gourronc and I. Housen et al., 1999. Purification and characterization of Aspergillus ficuum endoinulinase. Biosci. Biotecnol. Biochem., 63: 146-151.
    Direct Link    

    • © Science Alert. All Rights Reserved