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Journal of Biological Sciences

Year: 2005 | Volume: 5 | Issue: 3 | Page No.: 274-278
DOI: 10.3923/jbs.2005.274.278
Construction of a New Shuttle Vector for Acetobacter xylinum and Escherichia coli
Abbas Rezaee, Majid Ansari and Fathemeh Zandi

Abstract: Shuttle vector is a DNA molecule (e.g. plasmid) that is able to replicate in two different host organisms and can therefore be used to shuttle or convey genes from one to the other. In this study, a new shuttle vector was constructed that can replicate autonomously in Acetobacter xylinum and Escherichia coli. The new plasmid shuttle vector, designated pTMU30, was constructed from E. coli plasmid pUC18 and origin of replication (ori) of a cryptic plasmid pTMU20, which was originally isolated by us from a naturally occurring cellulose producing Acetobacter sp. The resulting shuttle vector, harboured resistance gene for ampicillin and capable of replicating in Acetobacter xylinum and E. coli. Transformation and replication of shuttle vector confirmed with antibiotic resistant experiments in the transformants bactrium. The vector was successfully transformed in Acetobacter xylinum and E. coli. This shuttle vector might used as a basis for genetic manipulation studies of Acetobacter xylinum.

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How to cite this article
Abbas Rezaee, Majid Ansari and Fathemeh Zandi, 2005. Construction of a New Shuttle Vector for Acetobacter xylinum and Escherichia coli. Journal of Biological Sciences, 5: 274-278.

Keywords: Acetobacter xylinum, E.coli, shuttle vector, construction and plasmid

REFERENCES
  • Umeda, Y., A. Hirano and M. Ishibashi, 1999. Cloning of cellulose synthase genes from Acetobacter xylinum JCM 7664. DNA Res., 6: 109-115.

  • Saxena, I.M., K. Kudlicka and R.M. Brown, 1994. Characterization of genes in the cellulose synthesizing operon (acs operon) of Acetobacter xylinum implications for cellulose crystallization. J. Bacteriol., 176: 5735-5752.
    PubMed    

  • Tonochi, N., N. Tahara and Y. Kojima, 1997. A beta glucosidase gene downstream of the cellulose synthase operon in cellulose producing Acetobacter. Biosci. Biotechnol. Biochem., 61: 1789-1790.

  • Lynd, L.R., P.J. Weimer, W.H. van Zyl and I.S. Pretorius, 2002. Microbial cellulose utilization: Fundamentals and biotechnology. Microbiol. Mol. Biol. Rev., 66: 506-577.
    CrossRef    PubMed    Direct Link    

  • Kawano, S., K. Tajima, Y. Liemor, H. Yamashit and T. Erata, 2002. Cloning of cellulose synthesis related genes from Acetobacter xylinum ATCC 23769 and ATCC 53582. DNA Res., 9: 149-156.

  • Tahara, N., H. Yaho and F. Yoshinaga, 1997. Two types of cellulase activity produced by a cellulose producing A strain. J. Fermentat. Bioeng., 83: 389-392.

  • Vieira, J. and J. Messing, 1982. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene, 19: 259-268.

  • Norrander, J., 1983. Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis. Gene, 26: 101-106.

  • Yanisch-Perron, C., J. Vieira and J. Messing, 1985. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors. Gene, 33: 103-119.
    PubMed    

  • Hestrin, H. and M. Schramm, 1954. Synthesis of cellulose by Acetobacter xylinum. Biochem. J., 58: 345-352.

  • Chrtyk, T.O., R.F. Sevensin, H.C. Cutler and V.A. Siesson, 1993. Methods for dillution antimicrobial suceptibility tests for bacteria that grow aerobically. Biosci. Biotechnol. Biochem., 57: 1355-1356.

  • Birnboim, H.C. and J. Doly, 1979. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res., 7: 1513-1523.
    CrossRef    PubMed    Direct Link    

  • Weng, S.F., N.T. Lin, Y.F. Fan, J.W. Lin and Y.H. Tseng, 1996. Characterization of the 1.8kb plasmid pXV64 from Xanthomonas campestris. Bot. Bull. Acad. Sin., 37: 93-98.

  • Friedman, A.M., S.R. Long, S.E. Brown, W.J. Buikema and F.M. Ausubel, 1982. Construction of a broad host range cosmid cloning vector and its use in the genetic analysis of Rhizobium mutants. Gene, 18: 289-296.

  • Valla, S., D.H. Coucheron and J. Kjosbakkeng, 1987. The plasmids of Acetobacter xylinum and their interaction with the host chromosome. Mol. Gen. Genet., 209: 412-415.

  • Chambers, S.P., S.E. Prior, D.A. Barstow and N.P. Minton, 1988. The pMTL nic-cloning vectors, I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing. Gene, 68: 139-149.

  • Sambrook, J., E. Fritsch and T. Maniatis, 2001. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, New York

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