Abstract: In this research, in vitro studies were realized on the Capsicum annuum L. varieties which is very important crop for Turkey. Capsicum anuum L. seedlings were grown from seeds which surfaced sterilized with 70% ethanol for 4 min 10% NaOCl (household bleach) solution for 6 min. The seeds were sown on the surface of hormone free MS (Murashige-Skoog) medium, pH adjusted to 5.8, the medium was solidified with 0.8% of bactoagar and 3% of sucrose used for carbon and energy source. After that, the cultures of the seeds germinated under 16 h photoperiod at 25°C. Cotyledone and hypocotyl explants from 3 weeks old seedlings were excised approximately 1 cm length segments. These explants were cultured on MS basal medium for callus formation. For determination to find what is the effects of drought stress or lowering osmotic potential conditions in the medium on the callus formation and response of the biomass induction. Polyethyleneglycol (PEG 3350) in 10% concentration were added to the MS medium and then biomass measurement were realized in 3 weeks interval from the callus. Two cultivars which belonging to the variete grossum (bell peppers) showed different responses against to the drought stress conditions. In the normal MS medium, biomass formation was very fast but when PEG used for changing the osmotic potential of the MS medium the biomass formation decreased because of the drougt stress conditions, after that MS medium was supplemented with auxines (NAA) and cytokinins (KIN) alone and together with PEG 3350 for recovering the biomass formation. As a result, auxines (NAA) and cytokinins (KIN) showed different recovering levels in two cultivars when they used alone or together. These growth regulators can use for to manage the drought stress conditions in the field studies before sowing or germination and this can be help to increase the plenty and productivity of some varieties for Capsicum annuum against drougt stress.