Abstract: For the first time, Momordica charantia L. was cultivated in Hofuf, Saudi Arabia. Fruits and leaves of the plant were analyzed for protein, vitamin C, total chlorophyll, chlorophyll A, chlorophyll B, chlorophyll A and B, nitrogen, phosphorus, potassium, copper, iron, manganese and zinc. Other cultivation was performed with NPK (20% nitrogen, 20% phosphorous, 20% potassium) fertilizer and the same constituents were determined. Effect of fertilization on level of constituents was examined. The obtained results are comparable with that of the plant cultivated in other areas in the world, while others are very lower. The study concluded that the nutritional contents in the edible parts of Momordica charantia L. can be improved by using NPK fertilizer during cultivation and plantation. Also, the study revealed that the fruit of the Saudi plant is highly nutritious.