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Journal of Biological Sciences

Year: 2004 | Volume: 4 | Issue: 4 | Page No.: 463-469
DOI: 10.3923/jbs.2004.463.469
Evaluation of Hepatoprotective Effects of Raphanas sativus L.
Rifat-uz-Zaman . and Mobasher Ahmad

Abstract: The hepatoprotective study of Raphanas sativus fruit powder (RS), it`s aqueous (RS-A) and ethanolic (RS-E) extracts was performed on rabbits. The fruit powder (0.5-1.5 g kg-1 of body weight) tested in cadmium chloride (Cd) treated rabbits. The effects of aqueous and ethanolic extracts of RS (equivalent to 1.5 g kg-1 of body weight) were also noted (with Cd). Both RS-A and RS-E significantly decreased the total bilirubin level and activity of enzymes i.e. ALT and AP in serum of Cd-treated rabbits. RS-E declined the serum ALT activity more effectively than the RS or RS-A in the treated animals. The lowering effect of the RS was more marked as compared to its extracts on the serum AP and total bilirubin level in the treated rabbits. Anti-inflammatory/anti-apoptosis activity of RS may be responsible for this hepatoprotective activity.

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How to cite this article
Rifat-uz-Zaman . and Mobasher Ahmad, 2004. Evaluation of Hepatoprotective Effects of Raphanas sativus L.. Journal of Biological Sciences, 4: 463-469.

Keywords: Raphanas, sativus, hepatoprotective, hepatitis, ALT (GPT), AP and bilirubin

REFERENCES
  • Sipos, P., K. Hasgymasi, A. Lugasi, E. Feher and A. Blazovics, 2002. Effects of black radish root (Raphanus sativus L. var niger) on the colon mucosa in rats fed a fat rich diet. Phytother. Res., 16: 677-679.

  • Murty, S., S.S. Va and S.N. Subrabmanyam, 1989. A Text Book of Economic Botany. Wiley Eastern Ltd., New Dehli, India, pp: 649-650.

  • Fant, F., W. Vranken, W. Broekaert and F. Borremans, 1998. Determination of the thee-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR. J. Mol. Biol., 29: 257-270.

  • Terras, F., H. Schoofs, K. Thevissen, R.W. Osborn, J. Vanderleyden, B. Cammue and W.F. Broekaert, 1993. Synergistic enhancement of the antifungal activity of wheat and barley thionins by radish and oilseed rape 2S albumins and by barley trypsin inhibitors. Plant Physiol., 103: 1311-1319.
    Direct Link    

  • Tawfik, N.I., B.A.H. El-Tawil, A.H. El-Refai, A.A. Khalaf and A.M. Khalil, 1978. Constituents of local plants. Part 1. Chemical investigations on some cultivated Saudi Arabian plants. Qual. Plant Foods Hum. Nutr., 28: 203-210.

  • CDC., 1990. Recommendations of the Immunization Practice Advisory Committee: Protection against viral hepatitis. MMWR., 39: 8-22.

  • Sorba, G., A. Gasco, G. Coruzzi, M. Adami, C. Pozzoli, G. Morini and G. Bertaccini, 1997. Mixed antisecretory and gastroprotective activities of a new H2-antagonist containing a nitric oxide-donor furoxan moiety. Arzneimittel-Forschung, 47: 849-854.
    PubMed    Direct Link    

  • Ishizuka, Y., T. Kamisai and M. Sato, 1996. Anti-gastric acid secretory mechanism of 1,6-dihydro-2-[2-(2-methylpropoxy)anilino]-6-oxo-5-pyrimidinecarboxylic acid. Effect on mucosal mast cell. Arzneimittel-Forschung, 46: 919-922.
    PubMed    Direct Link    

  • Sckiguchi, H., K. Hamada, H. Aijima, F. Taga, H. Uchida and K. Nishino, 1993. Healing-promoting action of the new histamine H2-receptor n-ethyl-[3-[3-(piperidinomethyl) phenoxy] propyl] urea with dual action on chonic gastric and duodenal ulcers induced by acetic acid in rats. Arzneium-Forsch/Drug Res., 43: 139-143.

  • Xiao-Bo, S., M. Tsukasa and Y. Haruki, 1992. Purification of anti-ulcer polysaccharide from the leaves of Panax ginseng. Planta Medica, 58: 445-448.

  • Akhtar, M.S. and M. Munir, 1989. Evaluation of the gastric anti-ulcerogenic effects of Solanum nigram, Brassica oleracea and Ocimum basilicum in rats. J. Ethnopharmacol., 27: 163-176.
    PubMed    Direct Link    

  • Rifat-uz-Zaman, 2004. Study of cardioprotective activity of Raphanus sativus L. in the rabbits. Pak. J. Biol. Sci., 7: 843-847.
    CrossRef    Direct Link    

  • Basinger, M.A., M.J. Mark, D.C. Warren, M. Ernest, J. Walker and S. Melinda, 1987. Chelating-agent suppression of cadmium-induced hepatotoxicity. Toxicol. Environ. Helth, 22: 261-272.

  • Harstad, E.B. and C.D. Klaassen, 2002. Gadolinium chloride pretreatment prevents cadmium chloride-induced liver damage in both wild-type and MT-null mice. Toxicol. Applied Pharmacol., 180: 178-185.

  • Rick, W., 1977. Method for Determination of Alkaline Phosphatase. Klinsche Chimie Mikroskopie Sringer Verlag, Berlin, 5: 242-242.

  • Miller, M.G., F.P. Veronica and J.D. David, 1986. Poisonous susceptibility of the fasted hamster to acetaminophen hepatotoxicity. Biochem. Pharmacol., 35: 817-826.

  • Dobbs, N.A., C.J. Twelves, W. Gregory, C. Cruickshanka, M.A. Richards and R.D. Rubens, 2003. Epirubicin in patients with liver dysfunction. Development and evaluation of a novel dose modification scheme. Eur. J. Cancer., 39: 580-586.

  • Xu, Q., Z. Lu and X. Zhang, 2002. A novel role of alkaline phosphatase in protection from immunological liver injury in mice. Liver, 22: 8-14.

  • Sauer, J.M., M.P. Waalkes, S.B. Hooser, A.T. Baines, R.K. Kuester and I.G. Sipes, 1997. Tolerance induced by all-trans-retinol to the hepatotoxic effects of cadmium in rats: Role of metallothionein expression. Toxicol. Applied Pharmacol., 143: 110-119.
    CrossRef    PubMed    Direct Link    

  • Sauer, J.M., M.P. Waalkes, S.B. Hooser, R.K. Kuester, C.A. McQueen and I.G. Sipes, 1997. Suppression of Kupffer cell function prevents cadmium induced hepatocellular necrosis in the male Sprague-Dawley rat. Toxicology, 121: 155-164.

  • Greabu, M. and R. Olinescu, 2002. The formation of oxidative stress condition in the experimental chemically induced hepatotoxicity. Rocz. Akad. Med. Bialymst, 47: 86-94.

  • Marinho, R., R. Pinto, M.L. Santos and M. De, 2002. Evidence for prostaglandin-producing supressor cells in HCV patients with normal ALT. Dig. Dis. Sci., 47: 556-561.

  • Fuente, D., P. Perez, Baranda, D. Barriga, S. Alanis, Layseca and G. Amaro, 2002. Effect of arsenic, cadmium and lead on the induction of apoptosis of normal human mononuclear cells. Clin. Exp. Immunol., 129: 69-77.
    CrossRef    PubMed    Direct Link    

  • Chuang, S.M., I.C. Wang, Y.S. Hwua and J.L. Yang, 2003. Short-term depletion of catalase suppresses cadmium-elicited c-Jun N-terminal kinase activation and apoptosis: Role protein phosphatases. Carcinogenesis, 24: 7-15.

  • Ferre, N., J. Camps, M. Cabre, A. Paul and J. Joven, 2001. Hepatic paraoxonase activity alterations and free radical production in rats with experimental cirrhosis. Metabolism, 50: 997-1000.

  • Hoglen, N.C., B.P. Hirakawa, C.D. Fisher, S. Weeks and A. Srinivasan et al., 2001. Characterization of the caspase inhibitor IDN-1965 in a model of apoptosis-associated liver injury. J. Pharmacol. Exp. Ther., 297: 811-818.

  • Xue, F., T. Takahara, Y. Yata, M. Minemura and C.Y. Morioka et al., 2002. Attenuated acute liver injury in mice by naked hepatocytes growth factor gene transfer into skeletal muscle with electroporation. Gut, 50: 558-562.

  • Liaw, W.J., C. Tzao, J.Y. Wu, S.J. Chen, J.H. Wang and C.C. Wu, 2003. Inhibition by terbutaline of nitric oxide and superoxide anion levels of endotoxin-induced organs injury in the anesthetized rat. Shock, 19: 281-288.

  • Vitoria, A.P., P.J. Lea and R.A. Azevedo, 2001. Antioxidant enzymes responses to cadmium in radish tissues. Phytochemistry, 57: 701-710.
    CrossRef    Direct Link    

  • Takaya, Y., Y. Kondo, T. Furukawa and M. Niwa, 2003. Antioxidant constituents of radish sprout (Kaiware-daikon), Raphanus sativus L. J. Agric. Food Chem., 51: 8061-8066.
    CrossRef    

  • Kirtikar, K.R and B.D. Basu, 1987. Indian Medicinal Plants. 2nd Edn., International Book Distributors, New York, pp: 178-180

  • Henry, R.J., 1964. Method for Determination of Total Bilirubin. Harper and Row, Inc., New York

  • Reitman, S. and S. Frankel, 1957. A colorimetric method for the determination of serum glutamic oxalacetic and glutamic pyruvic transaminases. Am. J. Clin. Pathol., 28: 56-63.
    CrossRef    PubMed    Direct Link    

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