Genotypic Differences of Two Wheat Cultivars for the Enzymes Activity, Amino Acids and Protein Profile under Fe Deficiency
Abstract:
A pot experiment was carried out at the National Research Centre, Dokki, Cairo-Egypt
using water culture technique. The possibility of using some biochemical and molecular
markers as a tool for the diagnosis of iron deficiency was studied in two bread wheat
cultivars (Triticum aestivum L.) cv, Sids I and Giza 164 which were different in their
response to iron deficiency. When iron was supplied at two levels (0 and 80 µM Fe-EDTA) the results indicated that, at 15 days old, the concentration of active iron (ferrous
form) in leaves were closely correlated with visual iron deficiency symptoms of both
cultivars. Generally chlorophylls concentration in leaves of both cultivars were decreased
in deficient plant compared to sufficient one. On the other hand a distinct differences in
the amount and types of amino acids was found in both cultivars whereas the total
amount of amino acids of chlorotic leaves were higher than non chlorotic one in Giza
164 cultivar. Activities of peroxidase and catalase enzymes in leaves were also
determined. Fe deficiency treatment resulted in decrease enzyme activities. Furthermore
the profile of isoperoxidase and proteins were modified in leaves and roots of both
cultivars during stress condition as evident from the electrogram.
How to cite this article
Amal A. Mohamed, F.K. El- Baz and R.K.H.M. Khalifa , 2003. Genotypic Differences of Two Wheat Cultivars for the Enzymes Activity, Amino Acids and Protein Profile under Fe Deficiency. Journal of Biological Sciences, 3: 864-874.
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