HOME JOURNALS CONTACT

Journal of Biological Sciences

Year: 2003 | Volume: 3 | Issue: 8 | Page No.: 751-759
DOI: 10.3923/jbs.2003.751.759
In vitro Plant Regeneration from Cotyledon-derived Callus of Three Varieties Pummelo (Citrus grandis [L.] Osb.)
F. Begum, M.N. Amin , S. Islam , M.A.K. Azad and M.M. Rehman

Abstract: Cotyledon explants from in vitro grown seedlings were cultured on half-strength of MS medium with different growth regulators for in vitro indirect regeneration of shoots. Optimum callus formation, when cotyledon explants were cultured on MS containing 1.0 mg l-1 BAP with 5.0 mg l-1 NAA. After that the calli were used for shoot regeneration by transferring them half-strength MS medium supplemented with only cytokinin. Maximum percentage of shoot regeneration was obtained on half strength MS medium in the presence of 1.0 mg l-1 BAP from callus in three varieties [Var.-1 (pink colour), Var.-2 (white colour) and Var.-3 (red colour)] of pummelo. For rooting, shoot cuttings were cultured on half strength MS salts with 0.1-1.0 mg l-1 NAA, IBA or IAA. The best and healthy rooting was observed on 0.1 mg l-1 NAA. The plantlets of three varieties were successfully established on soil. About 95% of plantlets survived under ex vitro condition.

Fulltext PDF

How to cite this article
F. Begum, M.N. Amin , S. Islam , M.A.K. Azad and M.M. Rehman , 2003. In vitro Plant Regeneration from Cotyledon-derived Callus of Three Varieties Pummelo (Citrus grandis [L.] Osb.). Journal of Biological Sciences, 3: 751-759.

Keywords: regeneration, In vitro, pummelo, citrus grandis and cotyledon

REFERENCES
  • Amin, M.N. and S. Akhtar, 1993. Regeneration of plants in vitro from seedling explants of pummelo (Citrus grandis [L.] Osb.). Plant Tissue Cult., 3: 71-79.

  • Baruah, A., V. Nagaraju and V.A. Parthasarathy, 1995. Cytokinin mediated response in shoots of Citrus grandis [L.]. Osb. Ann. Plant Physiol., 9: 13-16.

  • Begum, F., S. Islam and M.N. Amin, 2001. In vitro clonal propagation of pummelo (Citrus grandis [L.] Osb.). Plant Tissue Cult., 11: 141-149.

  • Bowman, K.D., 1995. Micropropagation of smooth flat Seville and Yuma citrus rootstocks. Fla. State Hortic. Soc. Meet. USA., 107: 15-18.

  • Carimi, F., F. De Pasquale and F.G. Crescimanno, 1999. Somatic embryogenesis and plant regeneration from pistil thin cell layers of Citrus. Plant Cell Rep., 11: 935-940.
    CrossRef    

  • Chaturevedi, H.C., A.R. Chowdhury and G.C. Mitra, 1974. Morphogenesis in stem callus tissue of Citrus grandis in long-term cultures: A biochemical analysis. Curr. Sci., 43: 139-142.

  • Chaturevedi, H.C., A.R. Chowdhury and G.C. Mitra, 1974. Shoot bud differentiation in stem callus tissue of Citrus grandis and correlated changes in its free amino acid content. Curr. Sci., 43: 536-537.

  • Duran-Vila, N., Y. Gogorcena, V. Ortega, J. Ortiz and L. Navarro, 1992. Micropropagation and tissue culture of sweet orange (Citrus sinensis [L.] Osb.): Effect of temperature and photosynthetic radiation. Plant Cell Tissue Organ Cult., 29: 11-18.

  • Grosser, J.W, J.L. Chandler, R.R. Pelosi and M. Cohen, 1994. In vitro micropropagation of seedless Cohen citrange. Florida State Hortic. Soci. Meet. USA., 106: 57-60.

  • Kantharajah, A.S., I. Dewitz and S. Jabbari, 1998. The effect of media, plant growth regulators and source of explants on in vitro culture of pomegranate (Punica granatum L.). Erwerbsobstbau, 40: 54-58.

  • Paudyal, K.P. and N. Haq, 2000. In vitro propagation of pummelo (Citrus grandis L. Osb.). In vitro Cell Dev. Biol. Plant, 36: 511-516.

  • Song, W.S., S.D. Oh and E.H. Park, 1991. In Vitro propagation of yooza (Citrus junos Sieb. et Tanaka). J. Korean Soc. Hort. Sci., 32: 206-215.

  • Prontip, W., 1993. Tissue Culture of Lime Citrus aurantifolia (Christm.) Swing. ADRC., Northeast, Thailand, pp: 2534-2535

  • Rahman, H., S.S. Gosal and S.S. Brar, 1987. Plant regeneration from callus culture of Citrus limon and C. jambhiri. Abst. Symposium on Crop Improvement, pp: 158-159.

    • © Science Alert. All Rights Reserved