Biochemical Evaluation of Combination Drug Interaction Between Phenobarbitone and Halofantrine in Weaning Rats
Abstract:
Combination drug interaction
between sodium phenobarbitone and halofantrine was studied biochemically
in weaning rats. Groups were made according to their body weights. All
the rats were sacrificed after 4 days of drug administration and the
liver homogenates and blood prepared for biochemical and haematological
investigations of cytotoxicity. Results showed that total protein contents,
monooxygenase, acid phosphatase and alkaline phosphatase activities
in the liver increased with increase in drug administered for all the
tests compared with the drug free controls. In the haematological analysis,
there was increase down the column with increase in drug for RBC, PCV
and Hb while the reverse was the case for WBC (total and differential)
compared with the controls. This would suggest that phenobarbitone had
an overwhelming sparing effect on the depletion of the haematological
parameters and induced their synthesis and lymphocytic maturation but
inhibited eosinophil production. The result indicated that phenobarbitone
affected the cytotoxic and haematological effects of halofantrine, an
antimalarial drug.
How to cite this article
H.C. C. Maduka, A. Salihu and V.A. Onwuliri, 2003. Biochemical Evaluation of Combination Drug Interaction Between Phenobarbitone and Halofantrine in Weaning Rats. Journal of Biological Sciences, 3: 711-717.
REFERENCES
Akintonwa, D.A.A., 1991. The wizardry of biochemistry in environmental toxicology and safety. Proc. Nig. Acad. Sci., 3: 87-97.
Akintonwa, D.A.A., 1992. The correlation between molecular acrobatic and biological effects of pyrimethamine. Nig. J. Biochem., 7: 62-66.
Akintonwa, D.A.A., N.E. Attah, E.U. Essien and C.Y.E. Aremu, 1993. Effect of a single or simultaneous administration of phenobarbitone and chloramphenicol on rat liver NADH-Nitroreductase activity. Nig. J. Biochem., 8: 23-27.
Essien, E.U., 1991. Effects of paracetamol and pyrimethamine administration on lipid components of selected tissues of the rats. Biosci. Res. Comm., 3: 183-190.
Maduka, H.C.C, C.O. Okere and P.C. Nwonu, 1993. Physicochemical evaluation of effects of phenobarbitone induction on anagol metabolism in weaning rats. Anns Borno, 10: 202-206.
Maduka, H.C.C., A. Salihu and V.A. Onwuliri, 2003. Biochemical evaluation of combination drug interaction between phenobarbitone and halofantrine in weaning rats. J. Biol. Sci., 3: 711-717.
CrossRef Direct Link
Maduka, H.C.C. and W.I. Mangzsha, 1997. Toxicological assessment of proguanil-phenobarbitone interaction: A biochemical study. West Afr. J. Biol. Sci., 6: 51-58.
Maduka, H.C.C., Z.S.C. Okoye, O. Ladeji and P.E. Egbe, 1999. The protective role of Sacoglottis gabonensis and bergen in, Nigeria alcoholic beverage additives against peroxidation reactions in vitro and in vivo. Nig. J. Biotech., 10: 1-8.
Umar, I.A., Z.G. Arjinoma, A. Gidado and H.H. Hamza, 1998. Prevention of garlic (Allium sativum Linn) induced anaemia in rats by supplementation with ascorbic acid and vitamin E. J. Biochem. Mol. Biol., 13: 31-36.
Umar, I.A., B.I. Madai, L.B. Buratai and Y. Karumi, 2002. The effect of combination of garlic (Allium sativum Linn) powder and wild honey on lipid metabolism in insulin-dependent diabetic rats. Nig. J. Exp. Applied Biol., 1: 37-42.
Goldsby, R.A., 1972. Experiments and Methods in Biochemistry. D.C Macmillan Publishing Co. Inc., New York, USA., pp: 55
Ibu, J.O. and O.K. Adeniyi, 1989. A Manual of Practical Physiology. Jos University Press Ltd., Jos, Nigeria, pp: 17-30
Jayaramen, J., 1981. Protein Content Determination in Practical Mammal in Biochemistry. Wiley, UK., pp: 78
Maduka, H.C.C. and A.A. Maduka, 2002. Biochemical and sociological approaches in drug abuse and safety a review. Ann. Borno, Vol. 19.
Mead, R. and R.N. Curnow, 1983. Statistical Methods in Agriculture and Experimental Biology. Chapman and Hall, London, United Kingdom, ISBN: 9780412242304, Pages: 335
Direct Link
Beecham, S.K., 1998. Package insert leaflet on halfan. Version 2.1. Smithkline Beecham Laboratories Pharmaceuticals, 6 Eaplanade Charles de Gaulle 92731, Nanterre, Cedex, France.
Stroev, E.A. and V.G. Makarowa, 1989. Monooxygenase assay. Laboratory Manual Biochem., pp: 233-237.
Verley, H., 1980. Assay of alkaline phosphatase. Practical Clin. Biochem., pp: 452-453.
Zimmermann, H.J., 1982. Chemical Hepatic Injury and Its Detection. In: Toxicology of the Liver, Plaa, G.G. and E.R. Hewitt (Eds.). Raven Press, New York, USA., pp: 1-45
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